TaqMan™ Environmental Master Mix 2.0
TaqMan™ Environmental Master Mix 2.0
Applied Biosystems™

TaqMan™ Environmental Master Mix 2.0

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0 offers accurate, real-time PCR-based pathogen detection in the presence of high levels ofRead more
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Catalog NumberNo. of Reactions
4396838200 Reactions
4398021400 Reactions
4398044800 Reactions
Catalog number 4396838
Price (CNY)
-
No. of Reactions:
200 Reactions
Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0 offers accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors. Use this Master Mix to analyze environmental, food and other challenging samples.

- Tailored for quantitative, real-time PCR experiments
- Unrivalled sensitivity for challenging applications, low copy number target detection and multiplex PCR
- Includes pathogen detection and viral⁄bacterial load quantification
- Sensitive detection for reliable quantification of abundant and limited genetic targets
- Stable mix for high-throughput handling

Optimized Formulation for Unrivalled Performance

TaqMan™ Environmental Master Mix 2.0 is a convenient 2X mix for target quantification that includes:

- AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure), a highly purified DNA Polymerase for improved detection of bacterial targets. This hot-start enzyme is inactive at room temperature so reactions can be set up on the benchtop. Enzyme is activated during thermal cycling.
- Passive internal reference based on proprietary ROX™ dye for precise data analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
FormatBottle
Hot StartBuilt-In Hot Start
No. of Reactions200 Reactions
Passive Reference DyeROX (Pre-mixed)
PolymeraseAmpliTaq Gold DNA Polymerase
Product LineTaqMan™
Product TypeqPCR Master Mix
Purity or Quality GradeUP (Ultra Pure)
Quantity200 reactions
Sample TypeEnvironmental Sample, Food
Shipping ConditionDry Ice
Sufficient For200 Reactions
Volume5 mL
Concentration2X
Detection MethodPrimer-probe
For Use With (Application)Gene Expression, Genotyping, Pathogen Detection, Virus Detection
PCR MethodqPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
This TaqMan Environmental Master Mix 2.0 is a convenient 2X mix in a 5 mL bottle that includes: AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), and a passive internal reference based on proprietary ROX dye for precise data analysis.
Upon receipt, store at -15 to -25°C before first use, protected from light. After first use, store at 2 to 8°C, protected from light.

Frequently asked questions (FAQs)

Is the 2X Environmental Master Mix the same as the TaqMan Universal PCR Master Mix, with or without UNG?

No, the 2X Environmental Master Mix (EMM) is not the same as the TaqMan Universal PCR Master Mix (with or without AmpErase UNG). The 2X Environmental Master Mix is specifically formulated to detect specific bacterial pathogens with greater specificity and sensitivity. It includes AmpliTaq Gold DNA Polymerase, LD, which is a highly purified version of AmpliTaq Gold DNA Polymerase. Every effort has been made in the development of this new polymerase to eliminate bacterial DNA from the enzyme preparation.

Is the TaqMan Environmental Master Mix 2.0 (Cat. No. 4398021, 4398044, 4396838) compatible with the QuantStudio instruments?

Yes, it is.

Find additional tips, troubleshooting help, and resources within our Gene Expression Analysis & Genotyping Support Center.

I have run out of Environmental Master Mix v2.0 from the Ion 16S Metagenomics Kit (Cat. No. A26216). Do you offer this as a standalone item?

Yes. You can purchase the TaqMan Environmental Master Mix 2.0 (Cat. No. 4396838).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center

How many PCR reactions can I perform using the 2X Environmental Master Mix within the Ion 16S Metagenomics Kit (Cat. No. A26216)?

It is sufficient for 100 PCR reactions.

Is there a mastermix that I can use with fecal samples?

For fecal samples, we recommend a master mix that is very tolerant of inhibitors, such as our TaqMan Environmental Master Mix 2.0. Please refer to the following paper: https://assets.thermofisher.com/TFS-Assets/CMD/posters/cms_089094.pdf