TaqMan™ 阵列人内源性对照品

Applied Biosystems™ TaqMan™ 表达内源性对照品板采用基于 TaqMan™ 探针的技术，专为 Applied Biosystems™ 实时荧光定量 PCR了解更多信息

货号	数量
4396840	1 standard plate

货号 4396840

价格（CNY)

4,105.00

添加至购物车

1 standard plate

价格（CNY)

4,105.00

添加至购物车

Applied Biosystems™ TaqMan™ 表达内源性对照品板采用基于 TaqMan™ 探针的技术，专为 Applied Biosystems™ 实时荧光定量 PCR 系统使用而设计–是定量基因表达的金标准，可提供更高的灵敏度、特异性、重现性和更广泛的动态范围。
任何研究基因表达相对定量的实验的第一步都应是内源性对照基因的选择，以便对样品输入中的变异进行均一化。TaqMan™ 表达人内源性对照品板含有32种基因，一式三份置于板中，且已证明这些基因是进行这类实验的良好候选基因。这组基因是从文献检索和⁄或在许多人类组织上进行的全基因组微阵列试验中挑选出来的。已证明它们在大多数待测样品中能够组成性地表达且丰度中等。

仅供科研使用。不可用于诊断程序。

产品规格96 孔板

内部探针修改MGB（小沟结合物）(3')、不发出荧光淬灭剂 (3')、FAM (5')

产品线TaqMan

纯化方法标准

数量1 standard plate

靶标管家基因&对照基因检测组合

适用于（应用）基因表达

标签或染料FAM

反应速度标准

Unit SizeEach

内容与储存

对照引物探针套件

常见问题解答 (FAQ)

What is the minimum amount of TaqMan array plates I need to purchase to submit an order?

The minimum amount of TaqMan Array plates needed to submit an order is based on the plate type:
• Inventoried TaqMan array plates: 1 plate
• TaqMan Flexible Content panel array plates: 6 plates
• Custom TaqMan array plates: 6 plates
• Specialty TaqMan array plates: 10-20 plates (depends on assay format and configuration). Please contact our Specialty Plates team at Specialty_Plates@thermofisher.com for details.

Can our TaqMan Array Plates be run on non-Applied Biosystem real-time PCR instruments?

We recommend using our Plastics Selection Tool to verify which plates are compatible with your instrument model, https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/plastics-selection-guide.html.

Based on our testing, the 96-well TaqMan Array Plates can be used on the following non-Applied Biosystem real-time PCR instruments:
• Standard (0.2mL) 96-well Plates: Bio-Rad IQ5
• Fast (0.1mL) Plates: Bio-Rad CFX Connect and Bio-Rad CFX96 Touch

How do I obtain TaqMan documentation files for my TaqMan array plates?

Beginning in April 2016, TaqMan documentation files for TaqMan Array Plates (assay information files (AIF), experimental setup files, and plate layout files) can be downloaded from the Thermo Fisher Scientific website at https://www.thermofisher.com/taqmanfiles. To obtain your TaqMan documentation files:
• Select the appropriate product type.
• Enter the information being requested for the product type.
• Fixed TaqMan array plates: Enter the Part number and Batch number
• TaqMan Flexible Content panel array plates/Custom TaqMan array plates: Enter the Sales Order number and Batch number
• Click Submit.

Can poly A+ purified RNA samples be used with the TaqMan Human Endogenous Control Plate (Cta. No. 4309199)?

No. The plate has been designed to evaluate only total RNA. Ribosomal RNA will be removed from poly A+ purified RNA samples, and is required for accurate detection of the 18s rRNA endogenous control assay used on the plate.

What types of templates can be used with the TaqMan Human Endogenous control Plate (P/N 4396840)?

The plate amplifies mRNA contained within total RNA. Non-human templates are not recommended, as all the assays are human specific with the exception of 18S rRNA and the internal positive control. Additionally, Poly A+ RNA cannot be accurately evaluated by the 18S rRNA assay as most of the rRNA has been removed.