A chemical cross-linking method for the analysis of binding partners of heat shock protein-90 in intact cells.
AuthorsSong S, Kole S, Bernier M,
JournalBiotechniques
PubMed ID22668512
'Members of the heat shock protein-90 (Hsp90) family are key regulators of biological processes through dynamic interaction with a multitude of protein partners. However, the transient nature of these interactions hinders the identification of Hsp90 interactors. Here we show that chemical cross-linking with ethylene glycolbis (succinimidylsuccinate), but not shorter cross-linkers, ... More