TaqMan™ Environmental Master Mix 2.0, 800 reactions - FAQs

查看更多产品信息 TaqMan™ Environmental Master Mix 2.0 - FAQs (4396838, 4398021, 4398044)

5 个常见问题解答

Is the 2X Environmental Master Mix the same as the TaqMan Universal PCR Master Mix, with or without UNG?

No, the 2X Environmental Master Mix (EMM) is not the same as the TaqMan Universal PCR Master Mix (with or without AmpErase UNG). The 2X Environmental Master Mix is specifically formulated to detect specific bacterial pathogens with greater specificity and sensitivity. It includes AmpliTaq Gold DNA Polymerase, LD, which is a highly purified version of AmpliTaq Gold DNA Polymerase. Every effort has been made in the development of this new polymerase to eliminate bacterial DNA from the enzyme preparation.

Is the TaqMan Environmental Master Mix 2.0 (Cat. No. 4398021, 4398044, 4396838) compatible with the QuantStudio instruments?

Yes, it is.

Find additional tips, troubleshooting help, and resources within our Gene Expression Analysis & Genotyping Support Center.

I have run out of Environmental Master Mix v2.0 from the Ion 16S Metagenomics Kit (Cat. No. A26216). Do you offer this as a standalone item?

Yes. You can purchase the TaqMan Environmental Master Mix 2.0 (Cat. No. 4396838).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center

How should I store TaqMan Environmental Master Mix 2.0 (Cat. No. 4398044), and how long can I store it after first use?

We recommend storing TaqMan Environmental Master Mix 2.0 (Cat. No. 4398044) at -20 degrees C, until first use. Once it is opened, it should be stored at 4 degrees C. You can store TaqMan Environmental Master Mix 2.0 until the expiration date listed on the CoA.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

Is there a mastermix that I can use with fecal samples?

For fecal samples, we recommend a master mix that is very tolerant of inhibitors, such as our TaqMan Environmental Master Mix 2.0. Please refer to the following paper: https://assets.thermofisher.com/TFS-Assets/CMD/posters/cms_089094.pdf