TaqMan™ Array Human MicroRNA A Card v2.0
TaqMan™ Array Human MicroRNA A Card v2.0
Applied Biosystems™

TaqMan™ Array Human MicroRNA A Card v2.0

TaqMan® Array Human MicroRNA A Card v2.0 包含 384 个了解更多信息
Have Questions?
货号数量
43989654 pack
货号 4398965
价格（CNY)
23,363.00
Each
添加至购物车
数量:
4 pack
价格（CNY)
23,363.00
Each
添加至购物车
TaqMan® Array Human MicroRNA A Card v2.0 包含 384 个 TaqMan® MicroRNA Assay，能够在 5 小时内对 377 个人类 microRNA 进行准确定量包含 3 种 TaqMan MicroRNA Assay 内参，可辅助数据标准化，还包括一种不与人类相关的 TaqMan® MicroRNA Assay 作为阴性对照。所包含的Assay的详细信息见目标列表文件。若运行该阵列，需要购买 Megaplex™ RT Primers，Human Pool A v2.1。当对实验灵敏度要求较高且⁄或样品有限时，可使用 Megaplex™ PreAmp Primers，Human Pool A v2.1 进行额外的预扩增步骤。
仅供科研使用。不可用于诊断程序。
规格
适用于（设备）AB 7900 HT 384 孔系统
形式干燥
内部探针修改MGB（小沟结合物）(3')，非荧光淬灭基团 (3')，FAM (5')
标签或染料FAM
产品线MegaPlex™，TaqMan™
数量4 pack
反应速度标准
研究类别基因调控，表观遗传学
类型阵列人 MicroRNA A 卡
产品规格384孔微流控卡
种属人
Unit SizeEach
内容与储存
4°C

常见问题解答 (FAQ)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.

View all TaqMan™ Array Human MicroRNA A Card v2.0 FAQs