Human cardiac tissue induces transdifferentiation of adult stem cells towards cardiomyocytes.
AuthorsPerán M, Marchal JA, López E, Jiménez-Navarro M, Boulaiz H, Rodríguez-Serrano F, Carrillo E, Sánchez-Espin G, de Teresa E, Tosh D, Aranega A,
JournalCytotherapy
PubMed ID20230311
'The goal was to induce the transdifferentiation (or conversion) of human adipose-derived stem cells to cardiomyocytes using an intracellular extract obtained from adult human heart tissue. Human adult stem cells from lipoaspirates were transiently permeabilized, exposed to human atrial extracts and allowed to recover in culture. After 21 days, the ... More
Impairment of peripheral circadian clocks precedes metabolic abnormalities in ob/ob mice.
AuthorsAndo H, Kumazaki M, Motosugi Y, Ushijima K, Maekawa T, Ishikawa E, Fujimura A,
JournalEndocrinology
PubMed ID21285316
'Recent studies have demonstrated relationships between the dysfunction of circadian clocks and the development of metabolic abnormalities, but the chicken-and-egg question remains unresolved. To address this issue, we investigated the cause-effect relationship in obese, diabetic ob/ob mice. Compared with control C57BL/6J mice, the daily mRNA expression profiles of the clock ... More
Using osteoclast differentiation as a model for gene discovery in an undergraduate cell biology laboratory.
AuthorsBirnbaum MJ, Picco J, Clements M, Witwicka H, Yang M, Hoey MT, Odgren PR,
JournalBiochem Mol Biol Educ
PubMed ID21567867
A key goal of molecular/cell biology/biotechnology is to identify essential genes in virtually every physiological process to uncover basic mechanisms of cell function and to establish potential targets of drug therapy combating human disease. This article describes a semester-long, project-oriented molecular/cellular/biotechnology laboratory providing students, within a framework of bone cell ... More