Megaplex™ PreAmp 引物，啮齿类动物池 Pool A
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Applied Biosystems™

Megaplex™ PreAmp 引物，啮齿类动物池 Pool A

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For use when assay sensitivity is of the utmost importance, Applied Biosystems® Megaplex™ PreAmp Primers significantly enhance the ability to了解更多信息
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货号数量
439920350 reactions
货号 4399203
价格（CNY)
11,934.00
Each
添加至购物车
数量:
50 reactions
价格（CNY)
11,934.00
Each
添加至购物车
For use when assay sensitivity is of the utmost importance, Applied Biosystems® Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. With content matched to the Megaplex™ RT Primers, Megaplex™ PreAmp Primers, Rodent Pool A is designed to be used with Megaplex™ RT Primers, Rodent Pool A. Following reverse transcription using Megaplex™ RT Primers, Megaplex™ PreAmp Primers along with the TaqMan® PreAmp Master Mix uniformly amplify all microRNAs prior to quantitation by real-time PCR using TaqMan® MicroRNA Arrays.
仅供科研使用。不可用于诊断程序。
规格
保真度（相对于 Taq）10 X
环保功能绿色环保包装
反应次数50 次反应
引物PreAmp 引物
产品线MegaPlex™，TaqMan™
产品类型Preamp 引物
数量50 reactions
运输条件室温
种属大鼠、小鼠
足够用于50 次反应
最大浓度10X
高 GC PCR 扩增效果低
PCR 方法qPCR
Unit SizeEach
内容与储存
含 150 μL 10X 引物

在 -15° 至 -25°C 下储存。

常见问题解答 (FAQ)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.

View all Megaplex™ PreAmp 引物，啮齿类动物池 Pool A FAQs