MegaPlex™ RT 引物，Human Pool A v2.1
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Applied Biosystems™

MegaPlex™ RT 引物，Human Pool A v2.1

Green features
Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis了解更多信息
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货号数量
439996650 reactions
货号 4399966
价格（CNY)
11,396.00
Each
添加至购物车
数量:
50 reactions
价格（CNY)
11,396.00
Each
添加至购物车
Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® MicroRNA Array. Human Pool A v2.1 contains RT primers for 377 unique microRNAs and 4 controls. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Human Pool B. Human Pool A v2.1 is intended for use with the TaqMan® Array, Human MicroRNA A Card v2.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.
仅供科研使用。不可用于诊断程序。
规格
环保功能绿色可持续包装
反应次数50 次反应
引物RT 引物
产品线MegaPlex™，TaqMan™
产品类型RT 引物
数量50 reactions
运输条件室温
种属人
足够用于50 次反应
适用于（应用）miRNA 分析
高 GC PCR 扩增效果高
PCR 方法qPCR
Unit SizeEach
内容与储存
储存在 -20°C 下。

常见问题解答 (FAQ)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.

View all MegaPlex™ RT 引物，Human Pool A v2.1 FAQs