Megaplex™ RT 引物，啮齿类动物池 A
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Applied Biosystems™

Megaplex™ RT 引物，啮齿类动物池 A

Green features
Designed to streamline experimental workflows, Applied Biosystems® Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time了解更多信息
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货号数量
439997050 reactions
货号 4399970
价格（CNY)
11,620.00
Each
添加至购物车
数量:
50 reactions
价格（CNY)
11,620.00
Each
添加至购物车
Designed to streamline experimental workflows, Applied Biosystems® Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® MicroRNA Array. Rodent Pool A contains RT primers for 335 and 238 unique microRNAs for mouse and rat respectively plus 4 species specific controls. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Human Pool A. The pool can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.
仅供科研使用。不可用于诊断程序。
规格
保真度（相对于 Taq）10 X
环保功能绿色可持续包装
反应次数50 次反应
引物RT 引物
产品线MegaPlex™，TaqMan™
产品类型RT 引物
数量50 reactions
运输条件室温
种属大鼠、小鼠
足够用于50 次反应
容量50 μL
最大浓度10X
高 GC PCR 扩增效果高
PCR 方法qPCR
Unit SizeEach
内容与储存
含有 50 μL 10X 引物和 1 mL 25 mM MgCl2。

在 -15° 至 -25°C 下储存。

常见问题解答 (FAQ)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.

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