Megaplex™ RT Primers, Rodent Pool A
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Applied Biosystems™

Megaplex™ RT Primers, Rodent Pool A

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Designed to streamline experimental workflows, Applied Biosystems® Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-timeRead more
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Catalog NumberQuantity
439997050 reactions
Catalog number 4399970
Price (CNY)
11,620.00
Each
Add to cart
Quantity:
50 reactions
Price (CNY)
11,620.00
Each
Add to cart
Designed to streamline experimental workflows, Applied Biosystems® Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® MicroRNA Array. Rodent Pool A contains RT primers for 335 and 238 unique microRNAs for mouse and rat respectively plus 4 species specific controls. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Human Pool A. The pool can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)10 X
Green FeaturesSustainable packaging
No. of Reactions50 reactions
PrimerRT Primers
Product LineMegaplex™, TaqMan™
Product TypeRT Primer
Quantity50 reactions
Shipping ConditionRoom Temperature
SpeciesRat, Mouse
Sufficient For50 Reactions
Volume50 μL
Concentration10X
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
Unit SizeEach
Contents & Storage
Contains 50μL of 10X primers and 1 mL 25 mM MgCl2.

Store at -15° to -25°C.

Frequently asked questions (FAQs)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.

View all Megaplex™ RT Primers, Rodent Pool A FAQs