TaqMan™ Copy Number Assay
TaqMan™ Copy Number Assay
Applied Biosystems™

TaqMan™ Copy Number Assay

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Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNARead more
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Catalog NumberQuantity
4400292M (750 reactions), made to order
4400291S (360 reactions), made to order
4400293L (2900 reactions), made to order
Catalog number 4400292
Price (CNY)
9,038.00
Each
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Quantity:
M (750 reactions), made to order

Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA targets using Applied Biosystems real-time PCR instruments and software. TaqMan Copy Number Assays are run together with a TaqMan Copy Number Reference Assay in a duplex qPCR reaction; the copy number assay detects the target sequence, and the reference assay detects a sequence that is known to be present in two copies in the diploid genome. The results are then analyzed by the relative quantitation method using Applied Biosystems CopyCaller Software.

The predesigned TaqMan Copy Number Assay collection consists of human and mouse assay libraries for copy number variation (CNV) analysis. The human assay collection includes over 1.6 million assays targeting gene exons and introns, extragenic regions, and CNV sequences from the Database of Genomic Variants (DGV). The mouse collection includes over 180,000 assays targeting gene exons. Assays to common vector marker and reporter genes are also available for transgenic studies.

Benefits:

  • Specific—gold-standard TaqMan chemistry with minor groove binder (MGB) probes increase assay specificity by enabling shorter probes
  • Reproducible—robust assay designs developed with our bioinformatics pipeline enable accurate, reproducible, and reliable results
  • Easy to interpret—CopyCaller Software provides the calculated copy number and predicted copy number, along with confidence value and z-score quality metrics
  • Fast and simple—setup to primary analysis in 3–4 hours

Approximate ship time

4–6 days in North America and 6–10 days in Europe

All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*

TaqMan Copy Number Reference Assays are sold separately.

Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355)

*Terms and conditions apply. For complete details, go to www.thermofisher.cn/taqmanguarantee.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
3'Primer ModificationNone
5'Primer ModificationNone
For Use With (Equipment)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Green FeaturesSustainable packaging
Internal Probe ModificationFAM (5'), MGB (Minor Groove Binder) (3')
No. of Reactions750 reactions
Product LineTaqMan™
Product TypeCopy Number Assay
Purification MethodSolid Phase Extraction (SPE)
Purity or Quality GradeRNase-Free, DNase-Free
QuantityM (750 reactions), made to order
Shipping ConditionRoom Temperature
SpeciesHuman
Volume750 μL
Concentration20X
Detection MethodPrimer-probe
FormFrozen
Label or DyeFAM
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

Frequently asked questions (FAQs)

Can I use TaqMan Gene Expression Assays for a gene copy number study?

TaqMan Gene Expression Assays with “_s1” as assay ID suffix are the assays whose primers and probes are designed within a single exon, and therefore, will detect genomic DNA. However, the application of these assays in gene copy number studies has not been validated. Other gene expression assays will not detect gDNA, let alone the region of variance.

What type of samples should be run with TaqMan Copy Number Assays?

These assays target regions of variance that are found in genomic DNA.

Do I need to do a validation test before I use the ΔΔCt method to determine the copy number of the target gene?

If you are using our copy number assays and reference assays, this is not necessary.

Where can I get reference samples for TaqMan Copy Number Assays?

We recommend that you check the DGV (database of genomic variants) database for your CNV of interest. Not all regions of variance have control samples available, but if there are controls for your CNV, the DGV will list some Coriell sample ids. You can then order these control samples from Coriell.

How many reactions can I get from each TaqMan Copy Number Assay?

TaqMan Copy Number Assay are available in 3 different sizes: small, medium and large. For the small size assays, they consist of two primers and a FAM-MGB TaqMan probe in a 20X formulation; enough for 360 - 20ul reactions. For more details, please see the TaqMan Copy Number Assays Protocol.

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