Custom TaqMan™ Copy Number Assay
Custom TaqMan™ Copy Number Assay
Applied Biosystems™

Custom TaqMan™ Copy Number Assay

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Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNARead more
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Catalog NumberQuantity
4400296L (2900 reactions), made to order
4400294S (360 reactions), made to order
4400295M (750 reactions), made to order
Catalog number 4400296
Price (CNY)
16,679.00
Each
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Quantity:
L (2900 reactions), made to order

Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA targets using Applied Biosystems real-time PCR instruments and software. TaqMan Copy Number Assays are run together with a TaqMan Copy Number Reference Assay in a duplex qPCR reaction; the copy number assay detects the target sequence, and the reference assay detects a sequence that is known to be present in two copies in the diploid genome. The results are then analyzed by the relative quantitation method using Applied Biosystems CopyCaller Software.

Custom TaqMan Copy Number Assays are an option for targets of interest that are not covered by the predesigned assay collection. The GeneAssist Copy Number Assay Tool enables you to submit your own premasked custom target sequences for assay design or primer/probe pair sequences for assay formulation, at no extra charge. The design process for Custom TaqMan Copy Number Assays does not include up-front bioinformatic sequence analysis or in silico QC, but the designs can be compared with the human/mouse reference assays for compatibility in duplex reactions.

Benefits:
• Flexible—generate assays for any target of interest using the GeneAssist Copy Number Assay Tool, with no species limitations
• Specific—gold standard TaqMan chemistry with minor groove binder (MGB) probes increase assay specificity by enabling shorter probes
• Reproducible—robust assay designs developed with our optimized algorithms deliver accurate, reproducible, and reliable results
• Easy to interpret—CopyCaller Software provides the calculated copy number and predicted copy number, along with confidence value and z-score quality metrics
• Fast and simple—setup to primary analysis in 3–4 hours

Approximate delivery time
4–6 days in North America and 6–10 days in Europe

TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

TaqMan Copy Number Reference Assays are sold separately.

Recommended master mix (sold separately): TaqMan Genotyping Master Mix

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)60 X
FormatTube
Green FeaturesSustainable packaging
No. of Reactions2900 reactions
Product LineTaqMan™
Product TypeCustom TaqMan™ Copy Number Assay
QuantityL (2900 reactions), made to order
Shipping ConditionRoom Temperature
Volume967 μL
Concentration60X
Detection MethodPrimer-probe
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

Frequently asked questions (FAQs)

Can I use TaqMan Gene Expression Assays for a gene copy number study?

TaqMan Gene Expression Assays with “_s1” as assay ID suffix are the assays whose primers and probes are designed within a single exon, and therefore, will detect genomic DNA. However, the application of these assays in gene copy number studies has not been validated. Other gene expression assays will not detect gDNA, let alone the region of variance.

What type of samples should be run with TaqMan Copy Number Assays?

These assays target regions of variance that are found in genomic DNA.

Do I need to do a validation test before I use the ΔΔCt method to determine the copy number of the target gene?

If you are using our copy number assays and reference assays, this is not necessary.

Where can I get reference samples for TaqMan Copy Number Assays?

We recommend that you check the DGV (database of genomic variants) database for your CNV of interest. Not all regions of variance have control samples available, but if there are controls for your CNV, the DGV will list some Coriell sample ids. You can then order these control samples from Coriell.

What TaqMan Copy Number Assays are available now?

We have predesigned TaqMan Copy number assays for human and mouse, and we also have the Geneassist Copy number assay workflow builder tool that you may use to submit sequences for custom designed assays. Copy number variation experiments require a reference assay run in duplex with the target assay. We offer RNase P and TERT reference assays for human, and we offer Tfrc and TERT reference assays for mouse. The copy number assays are FAM-MGB labeled and the copy number reference assays are VIC-TAMRA labeled.

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