Custom TaqMan™ 拷贝数检测试剂盒
Custom TaqMan™ 拷贝数检测试剂盒
Applied Biosystems™

Custom TaqMan™ 拷贝数检测试剂盒

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Applied Biosystems TaqMan 拷贝数检测试剂盒采用金标准 TaqMan MGB 探针化学试剂,使用 Applied Biosystems 实时了解更多信息
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货号数量
4400296967 μL
4400294360 μL
4400295750 μL
货号 4400296
价格(CNY)
16,679.00
Each
立刻订购
数量:
967 μL
Applied Biosystems TaqMan 拷贝数检测试剂盒采用金标准 TaqMan MGB 探针化学试剂,使用 Applied Biosystems 实时 PCR 仪器和软件来评估基因组 DNA 靶标的拷贝数。TaqMan 拷贝数检测试剂盒在双重 qPCR 反应中与 TaqMan 拷贝数参比检测试剂盒一起运行;拷贝数检测试剂盒检测靶标序列,而参比检测试剂盒已知存在于二倍体基因组的两个拷贝中的序列。然后使用 Applied Biosystems CopyCaller 软件通过相对定量法分析结果。

Custom TaqMan 拷贝数检测试剂盒是检测预设测定采集不包含的关注靶标的可选方法。GeneAssist 拷贝数检测工具使您可以提交自己的预屏蔽定制靶标序列用于检测设计,或提交自己的引物/探针对序列用于测定配方,无需额外收费。Custom TaqMan 拷贝数检测试剂盒的设计过程不包括前期生物信息序列分析或计算机模拟质量控制,但这些设计可与人类/小鼠参比测定比较,以实现双重反应的兼容性。

优势:
灵活性—使用 GeneAssist 拷贝数检测工具生成针对任何关注靶标的测定,且无物种限制
特异性—金标准 TaqMan 化学检测及小沟结合物 (MGB) 探针通过使用更短的探针而增加测定的特异性
重现性—通过优化的算法开发的稳健检测设计可提供准确、可重现且可靠的结果
易于解读—CopyCaller 软件提供计算的拷贝数和预测的拷贝数,以及置信值和 Z 分数质量指标
快速而简便—从构建到获得初步分析结果只需3–4小时

大概交付时间
北美4–6天,欧洲6–10天

TaqMan 检测试剂盒已被超过40,000篇出版物引用,并且已受到超过350项专利的支持。

TaqMan 拷贝数参比检测试剂盒为单独出售。

推荐的预混液(单独出售):TaqMan 基因分型预混液

仅供科研使用。不可用于诊断程序。
规格
保真度(相对于 Taq)60 X
产品规格管装
环保功能绿色可持续包装
反应次数2900 reactions
产品线TaqMan™
产品类型Custom TaqMan™ 拷贝数检测
数量967 μL
运输条件室温
容量967 μL
最大浓度60X
检测方法引物-探针
PCR 方法qPCR
反应速度快速
Unit SizeEach
内容与储存
1 个含 20X(S 和 M 规格)或 60X(L 规格)预配制检测试剂(1 种探针和 2 种引物)混合物的管。

在 -15 至 -25°C 下储存。

常见问题解答 (FAQ)

Can I use TaqMan Gene Expression Assays for a gene copy number study?

TaqMan Gene Expression Assays with “_s1” as assay ID suffix are the assays whose primers and probes are designed within a single exon, and therefore, will detect genomic DNA. However, the application of these assays in gene copy number studies has not been validated. Other gene expression assays will not detect gDNA, let alone the region of variance.

What type of samples should be run with TaqMan Copy Number Assays?

These assays target regions of variance that are found in genomic DNA.

Do I need to do a validation test before I use the ΔΔCt method to determine the copy number of the target gene?

If you are using our copy number assays and reference assays, this is not necessary.

Where can I get reference samples for TaqMan Copy Number Assays?

We recommend that you check the DGV (database of genomic variants) database for your CNV of interest. Not all regions of variance have control samples available, but if there are controls for your CNV, the DGV will list some Coriell sample ids. You can then order these control samples from Coriell.

What TaqMan Copy Number Assays are available now?

We have predesigned TaqMan Copy number assays for human and mouse, and we also have the Geneassist Copy number assay workflow builder tool that you may use to submit sequences for custom designed assays. Copy number variation experiments require a reference assay run in duplex with the target assay. We offer RNase P and TERT reference assays for human, and we offer Tfrc and TERT reference assays for mouse. The copy number assays are FAM-MGB labeled and the copy number reference assays are VIC-TAMRA labeled.