Silencer™ GAPDH Positive Control siRNA, in vivo ready - FAQs

View additional product information for Silencer™ GAPDH Positive Control siRNA, in vivo ready - FAQs (4404025)

14 product FAQs found

What controls should I use for my in vivo siRNA studies?

We offer the following in vivo siRNA controls:

Invitrogen In Vivo Negative Control #1 siRNA (Cat. No. 4457287, 5nmol; 4459405, 50 nmol; 4457289, 250 nmol)
Invitrogen In Vivo GAPDH Positive control siRNA (Cat. No. 4457288, 5 nmol; 4459407, 50 nmol, 4457291, 250 nmol)
Invitrogen In Vivo Factor VII Positive control siRNA (Cat. No. 4459408, 50 nmol; 4457292, 250 nmol)
Silencer Select Negative Control No. 1 siRNA, in vivo ready (Cat. No. 4404020, 250 nmol)
Silencer GAPDH Positive Control siRNA, in vivo ready (Cat. No. 4404025, 250 nmol)

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How stable is Stealth siRNA, can I use it for in vivo research?

Stealth siRNA is markedly more stable in serum than unmodified siRNA. This increased stability is particularly beneficial in vivo where the RNAi may be exposed to more proteases. In collaboration with Intradigm, Stealth RNAi has been demonstrated to have activity in vivo when injected intra-tumorally.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How can I order in vivo siRNA duplexes?

Pre-designed Stealth RNAi and Ambion In Vivo siRNA can be ordered directly from our pre-designed siRNA search interface (https://www.thermofisher.com/us/en/home/life-science/rnai/introduction-to-in-vivo-rnai.html?open=faq#search) to target human, mouse, or rat genes.

Custom siRNA designs for in vivo experiments can be ordered from two interfaces, depending on the product format being requested: Custom Ambion In Vivo siRNA is ordered from the GeneAssist Custom siRNA Builder (https://www.thermofisher.com/order/custom-genomic-products/tools/sirna/), while Stealth RNAi is ordered using the BLOCK-iT RNAi Express for in vivo synthetics (https://rnaidesigner.thermofisher.com/rnaiexpress/inVivoService.jsp).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Do I need endotoxin testing for my in vivo RNAi duplexes?

It is recommended to reduce the chance of eliciting an immune response.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I want to order larger scales than the standard offering, whom do I contact?

Please contact rnairesearcher@invitrogen.com regarding large scale in vivo siRNA or fill out the quote request form (www.thermofisher.com/us/en/home/global/forms/custom-sirna-mirna-quote-request.html).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How should I deliver my in vivo RNAi molecules?

Several different approaches have been used for siRNA delivery, including various local delivery techniques and systemic delivery. We offer a lipid reagent, Invivofectamine 3.0 Transfection Reagent for in vivo siRNA systemic delivery. Learn more about this reagent here (https://www.thermofisher.com/us/en/home/life-science/rnai/introduction-to-in-vivo-rnai/invivofectamine-reagent.html).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Should I use siRNA or vectors for in vivo RNAi?

RNAi can be delivered using two different approaches-siRNA synthetic duplexes or siRNA expressed from plasmids or viral vectors (shRNA, miRNAi). siRNA are becoming the method of choice for the fast development of therapeutics. They are easy to use, easy to design, and easy to synthesize. siRNA can be rapidly identified and multiple genes can be targeted at the same time. With RNAi vectors, the expression will be steadier as a result of the possibility of stable integration of the plasmid into the genome, and they have the ability to target nondividing cells such as stem cells, lymphocytes and neurons. The drawbacks are the danger of oncogenic transformation from insertional mutagenesis, and unanticipated toxicity from long-term silencing of human genes and/or having high amounts of siRNA inside the cell (Grimm D. et al.: Nature 441: 537-541 (2006)).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I am planning on using siRNA for my in vivo experiments, what purity should they be?

For Stealth siRNA we recommend to select HPLC in vivo purity. For Invitrogen In Vivo siRNA, we recommend in vivo ready purity.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Should I use labeled or unlabeled siRNA for my in vivo siRNA experiments?

We have demonstrated that labeling Stealth and Silencer siRNA does not hamper their knockdown potency. An alternative approach is to mix unlabeled duplexes with labeled control duplexes; this method is more commonly used with in vivo siRNA, and allows progression to clinical research unhindered by questions about the possible effects of a label.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Should I use chemically modified duplexes for my in vivo siRNA experiments?

Chemically modified siRNA duplexes have a number of advantages over standard siRNA duplexes, including the minimization of off-target effects, enhanced stability, and reduced toxicity. For these reasons, chemically modified siRNA duplexes are recommended for in vivo siRNA experiments. For some pilot experiments, where the only aim is to determine biodistribution, unmodified siRNA and fluorescent controls are useful and slightly more cost-effective.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What are the benefits of using our Invitrogen In vivo siRNA?

- 100x more stable than unmodified siRNA - longer knockdown in vivo
- Compatible with any 21-mer sequence - no loss of potency
- Non-toxic - actually reduces siRNA sequence specific immune responses
- Enhanced specificity - up to 90% reduction in off target effects
- Both Pre-designed and Validated Invitrogen In Vivo siRNAs offered


Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What siRNA do you offer that I can use for my in vivo studies, and what are the differences between them?

Please see the different siRNA we offer for in vivo studies:

Ambion In Vivo Pre-designed siRNAs are designed using the Silencer Select algorithm and incorporate additional chemical modifications for superior serum stability (half life >5 hours at 37 degrees C in 90% mouse serum) with in vivo applications. Ambion In Vivo siRNAs are non-toxic and non-immunogenic in vitro (peripheral blood nononuclear cells; PBMC) and in vivo (mouse). In cell-based assays, Ambion In Vivo siRNAs exhibit potency and specificity equivalent to Silencer Select siRNAs.
Ambion In Vivo Custom Designed siRNAs are designed to your specified target, using the Silencer Select algorithm and Ambion In Vivo chemical modifications.
Ambion In Vivo Custom Synthesized siRNAs are synthesized with your sequence, with Ambion In Vivo chemical modifications for serum stability.

Our Ambion In Vivo siRNA can be ordered with the following purities:

Desalted: Desalted and analyzed by MALDI-TOF mass spectrometry.
HPLC: HPLC purified and analyzed by MALDI-TOF and analytical HPLC
In Vivo Ready : HPLC purified, analyzed by MALDI-TOF and analytical HPLC, dialyzed to remove salts, sterile filtered, and endotoxin tested

Use our GeneAssist tool to order your custom siRNA (http://www.thermofisher.com/order/custom-genomic-products/tools/sirna/).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How critical is it to include positive and negative controls in siRNA experiments?

A positive control siRNA such as the Silencer Select GAPDH siRNA will demonstrate efficient transfection and help to optimize transfection conditions. A negative control siRNA is necessary to serve as a baseline for gene expression.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What are the positive and negative controls I should use with my Silencer or Silencer Select siRNA experiment?

We offer several negative and positive controls for both Silencer and Silencer Select siRNA.

For Silencer siRNA positive control:
- Silencer Firefly Luciferase (GL2 + GL3) siRNA (Cat. No. AM4629, 5nmol)
- Silencer GAPDH siRNA (human) (Cat. No AM4605, 5nmol; AM4633, 40 nmol)
- Silencer GAPDH siRNA (human, mouse, rat) (Cat. No. AM4624, 5 nmol; AM4631, 40 nmol; AM4632, 5 x 40 nmol)
- Silencer GFP (eGFP) siRNA (Cat. No. AM4626, 5 nmol)
- Silencer KIF11 (Eg5) siRNA (human, mouse, rat) (Cat. No. AM4639, 5 nmol)

If working with Fluorescently labeled Silencer siRNA:
- Silencer Cy-3 labeled GAPDH siRNA (human, mouse, rat) (Cat. No. AM4649, 5 nmol)
- Silencer FAM-labeled GAPDH siRNA (human, mouse, rat) (Cat. NO. AM4650, 5 nmol)

If working with Silencer siRNA in vivo ready:
- Silencer GAPDH Positive Control siRNA, in vivo ready (Cat. No. 4404025, 250 nmol)

For Silencer siRNA negative controls:
- Silencer Negative Control No. 1 siRNA (Cat. Nos. AM4611, 5 nmol; AM4635, 40 nmol; AM4636, 5 x 40 nmol)
- Silencer Negative Control No. 2 siRNA (Cat. Nos. AM4613, 5 nmol; AM4637, 30 nmol)
- Silencer Negative Control No. 3 siRNA (Cat. No. AM4615, 5 nmol)
- Silencer Negative Control No. 4 siRNA (Cat. No. AM4641, 5 nmol)
- Silencer Negative Control No. 5 siRNA (Cat. No. AM4642, 5 nmol)

If working with Fluorescently labeled Silencer siRNA:
- Silencer Cy 3-labeled Negative Control No. 1 siRNA (Cat. No AM4621, 5 nmol)
- Silencer FAM-labeled Negative Control No. 1 siRNA (Cat. No. AM4620, 5 nmol)

If working with Silencer siRNA in vivo ready:
- Silencer in vivo ready (Cat. No. 4404021)

For Silencer Select siRNA Positive control:
- Silencer Select GAPDH Positive Control siRNA (Cat. No. 4390849, 5 nmol; 4390850, 40 nmol; 4404024, 250 nmol)
- Silencer Select MALAT1 Positive Control siRNA for non-coding siRNA (Cat. No. 4455877, 5 nmol)

For Silencer Select siRNA negative control:
- Silencer Select Negative Control No. 1 siRNA (Cat. No. 4390843, 5 nmol; 4390844, 40 nmol)
- Silencer Select Negative Control No. 2 siRNA (Cat. No. 4390846, 5 nmol; 4390847, 40 nmol)

If working with Silencer Select siRNA in vivo ready:
- Silencer Select Negative Control No. 1 siRNA, in vivo ready (Cat. No. 4404020, 250nmol)

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.