TaqMan™ OpenArray™ 基因分型预混液

Applied Biosystems™

TaqMan™ OpenArray™ 基因分型预混液

该 2X 浓缩混合物经过优化，以便使用 TaqMan™ OpenArray™ SNP 基因分型板进行 SNP 基因分型。仅供研究使用了解更多信息

货号	数量
4404846	1 kit

货号 4404846

价格（CNY)

6,661.00

添加至购物车

1 kit

价格（CNY)

6,661.00

添加至购物车

该 2X 浓缩混合物经过优化，以便使用 TaqMan™ OpenArray™ SNP 基因分型板进行 SNP 基因分型。

仅供研究使用。不适用于诊断用途。

仅供科研使用。不可用于诊断程序。

保真度（相对于 Taq）2 X

适用于（设备）OpenArray™

基因分型靶标药物代谢酶、SNP（已知）

热启动内置热启动

参比荧光染料ROX（预混）

聚合酶AmpliTaq Gold DNA 聚合酶

产品线OpenArray™，TaqMan™

产品类型基因分型预混液

数量1 kit

样品类型DNA

运输条件湿冰

技术SNP 基因分型（基于实时定量 PCR）

最大浓度2X

检测方法引物-探针

适用于（应用）基因分型

PCR 方法qPCR

反应速度标准

Unit SizeEach

内容与储存

包含 TaqMan™ OpenArray™ 基因分型预混液、AmpliTaq Gold™ DNA 聚合酶（超纯）、dNTP 和优化的混合成分。该包装含有一个 5 mL 瓶，足以用于 32 个板。储存在 2–8°C 下。

常见问题解答 (FAQ)

What are the cycling conditions for OpenArray off-line cycling on a ProFlex PCR System?

These instructions may be found on page 79 of the ProFlex PCR System User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0007697.pdf).

Reaction volume: 33 nL
Cover temperature: 70°C

94°C for 600 sec, ramp rate 1.3

50 cycles of:
99°C for 30 seconds, ramp rate 1.3
54°C for 21 seconds, ramp rate 1.1
57°C for 90 seconds, ramp rate 0.1

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What are the cycling conditions for OpenArray off-line cycling on a GeneAmp PCR System 9700?

These instructions may be found on page 5 of the Dual Flat Block GeneAmp PCR System 9700 User Guide (https://tools.thermofisher.com/content/sfs/manuals/cms_065075.pdf).

93°C for 10 min, ramp rate 100%

50 cycles of:
95°C for 45 sec, 84% ramp rate
94°C for 13 sec, 100% ramp rate
53°C for 2 min 14 sec, 44% ramp rate

25°C for 2 min, 100% ramp rate

Optional: Add a post-PCR overnight hold as shown below:
4°C for 99 min 59 sec, 100% ramp rate

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What additional accessories do I need to perform off-line cycling for QuantStudio OpenArray plates?

You need an OpenArray Plate Adaptor, Cat. No. 4472306. You do not need a compression pad. This part number works with both the GeneAmp PCR System 9700 and the ProFlex PCR System.
Please note that this part number does not have a product page on our website. To order, please call our customer service team at 800-955-6288, option 1, option 1.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How can I get additional throughput for the QuantStudio 12K Flex OpenArray System?

Additional OpenArray plates can be run off-line using a flat-block thermal cycler. The two thermal cyclers we recommend are the GeneAmp PCR System 9700 and the ProFlex PCR System.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.