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View additional product information for MagMAX™ DNA Multi-Sample Kit - FAQs (4413020)
14 product FAQs found
可以，请订购货号4489112
不能，出厂实验方案不能编辑，但是您可以使用“另存为”功能对其重命名，然后将它作为一个新的版本来编辑。
对于核酸提取，我们建议使用我们的MagMAX试剂盒和MagJET试剂盒。其它大小在0.5到10 µm间的磁珠应该也可以使用。但是，您需要自己用Thermo Scientific BindIt软件编写您自己的KingFisher实验方案，并自己验证/优化这一实验方案。
可以，可将样品分装。在进行下一步之前，设置程序使得磁头可以从多个孔内收集样本。确保不要过量加样。
可以，反应体积可以扩大，并且所有其它体积（例如洗涤体积）需要以相同倍数扩大。除非板的容积允许，我们强烈建议反应体积扩大不应超过2倍。
是的，所有MagMAX试剂盒都能用于KingFisher Flex 磁珠处理仪。
Yes, please order Cat. No. 4489112.
The difference between Proteinase K for MagMAX‐96 DNA Multi‐Sample Kit and Proteinase K for MagMAX DNA Multi‐Sample Ultra Kit is the brand. The former is from Invitrogen legacy and the latter is from Applied Biosystems legacy. Both products have an identical concentration of 100 mg/mL and can be used interchangeably.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center
The MagMAX beads will cause a loss of ~2-3 µL of solution when they are pulled from the solution, but this is a very small amount. Larger volume loss, such as 20µL, can be due to several factors:
• Low lab humidity combined with a heated elution can cause this amount of liquid to evaporate. In this case, we recommend increasing the starting elution volume. Since the nucleic acid is not evaporating this should not affect your overall yield.
• Issues with alignment of the instrument components could also result in loss of volume. If the head goes down too deep during mixing, it could pull droplets up along the side. This would reduce elution volume and you would also be more likely to see a reduction in yield. A similar issue would result if the turntable alignment was off on either side.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.
For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes, reaction volumes can be scaled up, and all other volumes (i.e. wash volumes) will have to be increased by the same fold difference. It is generally not recommended to scale up reaction volumes more than 2-fold unless the plate's volume capacity will allow it.
Yes, all MagMAX kits can be used with the KingFisher Flex Magnetic Particle Processor.