PrepSEQ™ 残留 DNA 样品制备试剂盒

对 Applied Biosystems® PrepSEQ® 残留 DNA 样品制备试剂盒进行优化以便从复杂的蛋白质、缓冲液和盐混合物中高效回收 DNA，而这些混合物是需要对残留宿主细胞系 DNA了解更多信息

货号	数量
4413686	100 preps

货号 4413686

价格（CNY)

12,420.00

添加至购物车

100 preps

价格（CNY)

12,420.00

添加至购物车

对 Applied Biosystems® PrepSEQ® 残留 DNA 样品制备试剂盒进行优化以便从复杂的蛋白质、缓冲液和盐混合物中高效回收 DNA，而这些混合物是需要对残留宿主细胞系 DNA 进行定量分析的典型样品。可从具有挑战性的基质中获得定量且一致的 DNA 回收率，包括浓度较高、pH 值较低或盐浓度较高的样品

仅供科研使用。不可用于诊断程序。

最终产品类型基因组 DNA 纯化

适用于（应用）杂质检测

高通量能力非高通量兼容性（手动）、自动化方案

反应次数100 次制备

产品线PrepSEQ™

数量100 preps

样品类型生物制药生产样品

样品体积100 μL

靶标基因组 DNA 纯化

测试时间2小时

分离技术磁珠

Unit SizeEach

内容与储存

包括用于 DNA 提取、残留 DNA 定量、100 反应的缓冲液、磁珠和酶

常见问题解答 (FAQ)

The magnetic beads, that are shipped with the PrepSeq Residual DNA Sample Preparation Kit (Cat. No. 4413686), are clumped together and are difficult to get back into suspension. How can I fix this?

The beads are known to clump due to precipitation of the salts in the solution. These are highly concentrated chaotropic salt solutions. The only way to get the salts and the beads back into the solution is by incubating them at 37 degrees C for at least 10 minutes or until the solution is homogenous. Intermittent high speed vortexing helps expedite the process. To get beads stuck to the lid back into solution, we recommend inverting the tube and using high speed vortexing in a pulsing manner.
If incubating at 37 degrees C does not fix the issue, we recommend incubating at 45 degrees C.

What amount of DNA can be recovered with the PrepSEQ Residual DNA Sample Preparation Kit (Cat. No. 4413686)?

With the above kit, up to 5 ng of DNA can be recovered per 30 µl magnetic beads used per reaction. PrepSEQ is specially designed to recover lower amounts of DNA (in ng to sub femto‐gram levels).