With the VetMAX-Gold BVDV PI Detection Kit, I am getting signal detection in the no-template control (NTC) reaction. Why is this?
This is likely due to PCR contamination. Here are some recommendations:
- Repeat the qRT-PCR reaction with fresh reagents and freshly decontaminated pipettors.
- Set up and run the qRT-PCR reaction in an area that is isolated from areas used for RNA purification and PCR product analysis.