VetMAX™-Plus 一步法 RT-PCR 预混液
VetMAX™-Plus 一步法 RT-PCR 预混液
Applied Biosystems™

VetMAX™-Plus 一步法 RT-PCR 预混液

For use with VetMAX™ Reagents and VetMAX™ Controls. The VetMAX™-Plus One-Step RT-PCR Kit is designed for robust amplification of animal了解更多信息
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货号数量
4415328100 reactions
货号 4415328
价格（CNY)
4,919.00
Each
添加至购物车
数量:
100 reactions
价格（CNY)
4,919.00
Each
添加至购物车
For use with VetMAX™ Reagents and VetMAX™ Controls. The VetMAX™-Plus One-Step RT-PCR Kit is designed for robust amplification of animal pathogen RNA targets using a rapid, single-tube, real-time, reverse transcription PCR (RT-PCR) strategy. The kit includes Xeno™ RNA Control, which serves as an internal positive control for RNA purification and RT-PCR amplification. RNA is reverse-transcribed into cDNA, and then your target is amplified using AmpliTaq Gold® DNA Polymerase on a real-time PCR system.
规格
适用于（设备）7500 Fast 系统,7500 系统
产品规格管装
热启动内置热启动
反应次数100 次反应
参比荧光染料ROX（预混）
聚合酶AmpliTaq Gold DNA 聚合酶
产品线VetMAX™
产品类型应用于动物健康的 PCR 试剂和检测试剂盒
数量100 reactions
逆转录酶ArrayScript™ UP
样品类型RNA
运输条件干冰
足够用于100 次反应
检测方法引物-探针
适用于（应用）基因表达
形式液体
高 GC PCR 扩增效果低
PCR 方法一步法 RT-qPCR
反应速度标准
Unit SizeEach
内容与储存
保存于 -20°C。包括含以下成份的100次反应的试剂：RT-PCR 缓冲液、RT-PCR 酶混合物、Xeno™ RNA 对照和无核酸酶的水。

常见问题解答 (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

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