Stability of an autoinhibitory interface in the structure of the tyrosine kinase ZAP-70 impacts T cell receptor response.
AuthorsDeindl S, Kadlecek TA, Cao X, Kuriyan J, Weiss A,
JournalProc Natl Acad Sci U S A
PubMed ID19920178
'The delivery of signals from the activated T cell antigen receptor (TCR) inside the cell relies on the protein tyrosine kinase ZAP-70 (zeta-associated protein of 70 kDa). A recent crystal structure of inactive full-length ZAP-70 suggests that a central interface formed by the docking of the two SH2 domains of ... More
T cell receptor signal initiation induced by low-grade stimulation requires the cooperation of LAT in human T cells.
AuthorsDong S, Corre B, Nika K, Pellegrini S, Michel F,
JournalPLoS One
PubMed ID21152094
'One of the earliest activation events following stimulation of the T cell receptor (TCR) is the phosphorylation of the immunoreceptor tyrosine-based activation motifs (ITAMs) within the CD3-associated complex by the Src family kinase Lck. There is accumulating evidence that a large pool of Lck is constitutively active in T cells ... More
Regulated vesicle fusion generates signaling nanoterritories that control T cell activation at the immunological synapse.
AuthorsSoares H, Henriques R, Sachse M, Ventimiglia L, Alonso MA, Zimmer C, Thoulouze MI, Alcover A,
Journal
PubMed ID24101378
How the vesicular traffic of signaling molecules contributes to T cell receptor (TCR) signal transduction at the immunological synapse remains poorly understood. In this study, we show that the protein tyrosine kinase Lck, the TCR? subunit, and the adapter LAT traffic through distinct exocytic compartments, which are released at the ... More
PALM imaging and cluster analysis of protein heterogeneity at the cell surface.
AuthorsOwen DM, Rentero C, Rossy J, Magenau A, Williamson D, Rodriguez M, Gaus K,
JournalJ Biophotonics
PubMed ID20148419
The authors employed photoactivatable localization microscopy (PALM) and direct stochastic optical reconstruction microscopy (dSTORM) imaging and image analysis based on Ripley's K-function to quantify the distribution and heterogeneity of proteins at the cell plasma membrane. The membrane targeting sequence of the N-terminal region of the T cell receptor-pathway kinase Lck ... More
Intramolecular regulatory switch in ZAP-70: analogy with receptor tyrosine kinases.
AuthorsBrdicka T, Kadlecek TA, Roose JP, Pastuszak AW, Weiss A,
JournalMol Cell Biol
PubMed ID15923611
ZAP-70, a Syk family cytoplasmic protein tyrosine kinase (PTK), is required to couple the activated T-cell antigen receptor (TCR) to downstream signaling pathways. It contains two tandem SH2 domains that bind to phosphorylated TCR subunits and a C-terminal catalytic domain. The region connecting the SH2 domains with the kinase domain, ... More