RNAlater和RNAlater ICE试剂可以重复使用吗?
我们不推荐重复使用RNAlater和RNAlater ICE试剂。
Are RNAlater Stabilization Solution and RNAlater-ICE Frozen Tissue Transition Solution chemically reactive with oxidizing agents?
RNAlater Stabilization Solution and RNAlater-ICE Frozen Transition Solution are known to react with hypochlorite solutions, such as common bleach. The reaction releases chlorine gas and generates heat. A similar reaction may occur with other oxidizing agents. If you suspect that samples may contain bleach or other oxidizing reagents, we recommend working in a fume hood with adequate protective clothing and equipment.
Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.
Can I add RNAlater reagent to samples that are already frozen?
A different product, RNAlater-ICE reagent, is used with samples that are already frozen. RNAlater-ICE reagent transitions tissue from a frozen to a non-frozen state. The frozen tissue is simply placed in RNAlater-ICE reagent and left at -20 degrees C overnight. Treated tissues can then be used directly in standard homogenization and isolation protocols and processed like fresh tissue.
RNAlater reagent and RNAlater-ICE reagent provide flexibility for sample collection and storage, and help ensure that high quality RNA is preserved in samples. Both are available in a variety of convenient sizes.
Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.