RNAlater™-ICE 冰冻组织过渡溶液
RNA<i>later</i>&trade;-ICE 冰冻组织过渡溶液
Invitrogen™

RNAlater™-ICE 冰冻组织过渡溶液

RNAlater-ICE 是一种新型试剂,可以将冷冻组织转变为一种易于处理以提取高质量 RNA 的状态。只需将冷冻组织浸入 RNAlater-ICE 中,然后,在 -20°C 下过夜解冻。解冻后,组织可像新鲜组织一样使用标准了解更多信息
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货号数量
442757510 x 25 mL
AM703025 mL
货号 4427575
价格(CNY)
5,850.00
Each
添加至购物车
数量:
10 x 25 mL
价格(CNY)
5,850.00
Each
添加至购物车
RNAlater-ICE 是一种新型试剂,可以将冷冻组织转变为一种易于处理以提取高质量 RNA 的状态。只需将冷冻组织浸入 RNAlater-ICE 中,然后,在 -20°C 下过夜解冻。解冻后,组织可像新鲜组织一样使用标准 RNA 分离程序进行处理。不再需要费力研磨冷冻组织,从而可保护 RNA。

• 像新鲜采集的样品一样处理之前冷冻的组织
• 在 RNAlater-ICE 中解冻组织可保护 RNA 免受降解
• 不再使用研钵和研杵粉碎组织,无需棘手地将粉末转移到管中
• 易于为多项实验分配冷冻组织样品
• 快速冷冻组织可保留 RNA

通常,需要在 RNA 分离前储存的组织均在干冰或液氮中“快速”或“急速”冷冻以保持 RNA 完整性。在 -80°C 下冷冻时,组织中的 RNA 可保持稳定,但在组织破坏之前或破坏期间解冻组织可导致 RNA 降解。即使组织在变性溶液中解冻,也是如此。

处理冷冻组织存在问题
冷冻组织通常使用冰研钵和研杵研磨,以维持 RNA 质量。必须向研钵中加入液氮,以使样品在研磨时保持冷冻。对于多份样品,该过程非常费力。每份样品都需要一套单独的研钵和研杵,或者在处理每份样品后必须解冻和清洁该套工具,以避免交叉污染。粉末组织也可在转移至均质化容器中时解冻。这通常会形成团块,这些团块在裂解液中不易于分散,从而导致 RNA 降解和丢失。非常小的样品应立即在裂解液中均质化,如果要处理多份样品,则可能较繁琐。

在不损害 RNA 完整性的情况下处理冷冻组织
RNAlater-ICE 可解决所有这些问题。只需将冷冻组织样品浸入 10 体积 RNAlater-ICE 中,并在 -20°C 下过夜储存(溶液在这些温度下仍为液体)。随着组织解冻,RNA 完整性得到保护。处理后,可在 4°C 或甚至室温下安全储存组织(有限时间),并可在均质化之前在标准 RNA 分离裂解缓冲液中进一步分离或处理组织。因此,同一冷冻组织样品可多次用于不同的实验,而不会损害 RNA 的完整性。

仅供科研使用。不适用于诊断用途。
仅供科研使用。不可用于诊断程序。
规格
描述RNAlater™ 稳定液
产品线RNAlater
产品类型RNA 储存试剂
数量10 x 25 mL
缓冲液组织提取缓冲液
Unit SizeEach

常见问题解答 (FAQ)

RNAlater和RNAlater ICE试剂可以重复使用吗?

我们不推荐重复使用RNAlater和RNAlater ICE试剂。

Is it possible to reuse RNAlater reagent and RNAlater ICE reagent?

We do not recommend reusing RNAlater reagent or RNAlater ICE reagent.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

Are RNAlater Stabilization Solution and RNAlater-ICE Frozen Tissue Transition Solution chemically reactive with oxidizing agents?

RNAlater Stabilization Solution and RNAlater-ICE Frozen Transition Solution are known to react with hypochlorite solutions, such as common bleach. The reaction releases chlorine gas and generates heat. A similar reaction may occur with other oxidizing agents. If you suspect that samples may contain bleach or other oxidizing reagents, we recommend working in a fume hood with adequate protective clothing and equipment.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

Can I add RNAlater reagent to samples that are already frozen?

A different product, RNAlater-ICE reagent, is used with samples that are already frozen. RNAlater-ICE reagent transitions tissue from a frozen to a non-frozen state. The frozen tissue is simply placed in RNAlater-ICE reagent and left at -20 degrees C overnight. Treated tissues can then be used directly in standard homogenization and isolation protocols and processed like fresh tissue.
RNAlater reagent and RNAlater-ICE reagent provide flexibility for sample collection and storage, and help ensure that high quality RNA is preserved in samples. Both are available in a variety of convenient sizes.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

How can I ship my sample in RNAlater reagent?

Samples in RNAlater reagent can safely be shipped on wet ice for several days. For longer shipping times use dry ice.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.