Region of Interest (ROI) and Background Plates, 96-well - FAQs

I want to purchase the ROI/Uniformity, background calibration and RNase P verification plates for the QuantStudio 5 Real Time PCR System, 96-well, 0.2 ml (Cat. No. A28139). What are the Catalog numbers for these plates?

The Catalog numbers are as follows:

• Region of Interest (ROI) and Background Plates, 96‐well (Cat. No. 4432364)
• TaqMan RNase P Instrument Verification Plate for ViiA 7 System, 96‐well (for 0.2 mL block) (Cat. No. 4432382)

I cannot open the QuantStudio 3/5 Design & Analysis 2 (DA2) software on my Mac. What are the software requirements for Mac?

To open the software download on your Mac, you will need to adjust your security settings using the following instructions from Apple support:

Safely open apps on your mac

Please note, the software should run on Mac OS 10.10 or later.

Find additional tips, troubleshooting help, and resources within our QuantStudio 3 and QuantStudio 5 Real Time PCR Systems Support Center.

What is the expiration date for the Region of Interest (ROI) and Background Plates, 96-well (Cat. No. 4432364)?

The expiration date for the Region of Interest (ROI) and Background Plates, 96-well (Cat. No. 4432364) is 12 months from the date of manufacturer, which can be found on the Certificate of Analysis (COA).

You can find the COA for a specific lot number by using the Documents and Certificatessearch page or by filling out a Certificate of Analysis (COA) or Certificate of Origin (COO) Request Form.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

How can I get a summary of the calibrations performed on the QuantStudio 6 and 7 Flex Real-Time PCR Systems?

You can get a summary of the calibrations from the QuantStudio Software by going to Instrument > Instrument Console > Manage Instrument > Information > Print Calibration Log.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Do I need to back up my calibration files before I upgrade the software for the ViiA7 Real-Time PCR System?

No, you do not need to back up the calibration files as all of the calibration files are stored on the ViiA7 Real-Time PCR instrument. Once the computer software is properly installed and the computer is connected to the instrument, the calibration information will automatically update in the QuantStudio Software.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I still start a run on the ViiA7 Real-Time PCR System if the "Calibration is expired" notification is displayed?

Yes, you can still start a run on the ViiA7 Real-Time PCR System if the expired calibration notice displays. This is just a reminder and you can close it to proceed with the run. Additionally, you can change when you want the notifications to show up and how frequently you want to be notified to calibrate the instrument. To do this, go to the toolbar in the QuantStudio Software and select Instrument > Instrument Console. Select your instrument icon and click on Manager Instrument > Maintenance > Calibration Reminders. From the Calibration reminders Settings page you can update when the calibrations expire, turn expiration reminders on/off, and set when the reminders show up.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.