When do I need to run the RNaseP calibration?
The RNaseP verification plate contains template, master mix, and a TaqMan assay for RNaseP. It is used to verify that the instrument is performing to specifications. If you have reason to suspect there is something wrong with the instrument, or if you want to rule out a chemistry issue, the RNaseP plate is a good way to test the system. The RNaseP verification plate is a single-use plate.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
When do I need to run the RNaseP calibration for the QuantStudio 12K Flex Real-Time PCR System?
The RNaseP verification plate contains template, master mix, and a TaqMan assay for RNaseP. It is used to verify that the instrument is performing to specifications. If you have reason to suspect there is something wrong with the instrument, or if you want to rule out a chemistry issue, the RNaseP plate is a good way to test the system. The RNaseP verification plate is a single-use plate.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
I want to purchase the ROI/Uniformity, background calibration and RNase P verification plates for the QuantStudio 5 Real Time PCR System, 96-well, 0.2 ml (Cat. No. A28139). What are the Catalog numbers for these plates?
The Catalog numbers are as follows:
- Region of Interest (ROI) and Background Plates, 96‐well (Cat. No. 4432364)
- TaqMan RNase P Instrument Verification Plate for ViiA 7 System, 96‐well (for 0.2 mL block) (Cat. No. 4432382)
For more information on calibration plates for the different Applied Biosystems Real Time PCR systems, please visit the following webpage.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
I cannot open the QuantStudio 3/5 Design & Analysis 2 (DA2) software on my Mac. What are the software requirements for Mac?
To open the software download on your Mac, you will need to adjust your security settings using the following instructions from Apple support:
Safely open apps on your mac
Please note, the software should run on Mac OS 10.10 or later.
Find additional tips, troubleshooting help, and resources within our QuantStudio 3 and QuantStudio 5 Real Time PCR Systems Support Center.
Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?
It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system
Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".
