I want to purchase TaqMan Array Human MicroRNA A Cards v2.0 (Cat. No. 4398965). What other reagents do I need to purchase in addition to these cards?
In order to perform reverse transcription on these cards, you will need the following:
- TaqMan MicroRNA Reverse Transcription Kit (Cat. No. 4366596)
- Megaplex RT Primers pools (for human Type A cards Cat. No. 4399966, for Type B cards Cat. No. 4444281)
You can proceed with or without a preamplification step which depends on the total RNA amount. Total RNA amount of 1‐1000 ng supports reverse transcription reaction with preamplification, whereas 350‐1000 ng of total RNA supports a reaction without preamplification.
In case you are performing preamplification you would also need the TaqMan PreAmp Master Mix (Cat. No. 4391128) and the Megaplex PreAmp Primers, Human Pool A v2.1 (Cat. No. 4399233).
You will also need the Master Mix for the qPCR reaction. It is not included in the card kit. The recommended Master Mix is: TaqMan Fast Advanced Master Mix – run mode Fast (Cat. No. 4444556)
For further information, please see the following
link.Find additional tips, troubleshooting help, and resources within our
Real-Time PCR and Digital PCR Applications Support Center.