TaqMan™ Universal Master Mix II, no UNG, 5 x 5 mL - FAQs

查看更多产品信息 TaqMan™ Universal Master Mix II, no UNG - FAQs (4440043, 4440041, 4440040, 4440047, 4440049, 4440048)

5 个常见问题解答

Do I have to use the Universal Master Mix with my TaqMan MicroRNA Assays? If so, which one?

Yes. This is the recommended master mix for TaqMan MicroRNA Assays. You can use Universal Master Mix or Universal Master Mix II (with or without UNG). You can also use the Fast Advanced Master Mix.

Is the 2X Environmental Master Mix the same as the TaqMan Universal PCR Master Mix, with or without UNG?

No, the 2X Environmental Master Mix (EMM) is not the same as the TaqMan Universal PCR Master Mix (with or without AmpErase UNG). The 2X Environmental Master Mix is specifically formulated to detect specific bacterial pathogens with greater specificity and sensitivity. It includes AmpliTaq Gold DNA Polymerase, LD, which is a highly purified version of AmpliTaq Gold DNA Polymerase. Every effort has been made in the development of this new polymerase to eliminate bacterial DNA from the enzyme preparation.

Is the TaqMan Fast Universal PCR Master Mix offered with AmpErase UNG?

The TaqMan Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpEraseUNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan Fast Advanced Master Mix.

Can I compare performance of assays between the TaqMan Fast Universal PCR Master Mix compare and TaqMan Universal PCR Master Mix?

In general, assays have been found to perform comparably with these reagents, but they may not provide exactly identical results.

What is the benchtop stability of the TaqMan miRNA Assay mixed with TaqMan Universal Master Mix II (Cat. No. 4440043)?

24 hours.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.