TaqMan Fast™ 病毒1步法预混液

可搭配久经验证的即用型TaqMan assay 进行基因表达、基因分型、或定制探针的进行多重检测。欢迎使用我们TaqMan Search Wizard帮您找到目标基因的合适Assay。点此了解更多信息

引用和文献 (959)

Applied Biosystems™

TaqMan Fast™ 病毒1步法预混液

Name: TaqMan Fast™ 病毒1步法预混液
SKU: 4444434
Price: 23677.00 CNY

TaqMan Fast 病毒一步法预混液专用于快速的高灵敏度实时 RT-PCR，即使存在具有挑战性的 PCR 抑制剂也适用。4X 制剂增强了对 RNA 和了解更多信息

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货号	数量
4444434	5 x 1 mL
4444436	1 x 10 mL
4444432	1 x 1 mL

3 选项

货号 4444434

价格（CNY)

23,677.00

Each

添加至购物车

数量:

5 x 1 mL

价格（CNY)

23,677.00

Each

添加至购物车

TaqMan Fast 病毒一步法预混液专用于快速的高灵敏度实时 RT-PCR，即使存在具有挑战性的 PCR 抑制剂也适用。4X 制剂增强了对 RNA 和 DNA 病毒的检测，是多通路基因表达研究的理想选择，即使靶标上样量较低也适用。TaqMan Fast 病毒一步法预混液采用单管形式以便统一处理和加工，同时在 <30 分钟内提供 qPCR 循环，这在高通量工作流程中运行时非常有用。

主要产品特点：
• 高灵敏度—4X 预混液以更高的灵敏度扩增 RNA 和 DNA
•• 一致结果—专门用于处理血液、粪便和其他难处理样品中常见的 RT-PCR 抑制剂
•• 简化的应用—单管一步法预混液，提供 RNA 和 DNA 单次运行分析，轻松实现孔板上靶标的混合和匹配
•• 靶标灵活性—能够以单通路或多通路形式工作，并可使用外源或内源性内参
•• 高效率—提高快速和标准仪器上 qRT-PCR 的速度

两种配方

该预混液有两种配方可供选择：

• 对于单通路（1个探针）和多达三通路（3个探针）反应，建议使用含有 ROX 染料（作为惰性参比染料）的 TaqMan Fast 病毒一步法预混液

• 不含惰性参比染料的 TaqMan Fast 病毒一步法多通路预混液（不含 ROX）推荐用于使用 JUN（或类似发射波长）染料的测定或用于更高阶的多通路（>3 个靶标）测定

高灵敏度
许多带有病毒核酸的常见研究样品中含有非常低水平的靶标。我们对预混物进行了优化，以对病毒靶标进行高灵敏度检测。高浓度预混物允许您构建较小的反应体积，这意味着您可以执行快速循环实验方案，并获得至少与标准循环 qPCR 相同的灵敏度。也可以将更大量样品添加至标准反应体积中，以更准确地定量低滴度样品（下图）。

在存在抑制剂的情况下获得一致结果
通常用于测定包含病毒的研究样品，包括血液、污垢以及组织样品。TaqMan Fast 病毒一步法预混液中的缓冲液组分和专有添加剂已经过优化，可对 RT-PCR 抑制剂进行处理（下图），即使处理复杂样品也能确保一致的性能，从而帮助您获得更可靠的结果。

靶标灵活性
在实验室中通常需要检测各种样品中的 RNA 和 DNA 病毒。为了简化您的实验，制定了单个 TaqMan Fast 病毒一步法预混液方案以测定这两种类型的核酸，因此您可以使用相同的处理步骤在同一孔板上相邻孔进行 RNA 和 DNA 病毒检测（下图）。此外，由于病毒研究通常包括多重引物和探针以及内部反应对照，我们对预混物进行了优化以与多种靶扩增子一起使用（下图）。

快速获得结果
TaqMan Fast 病毒一步法预混液有助于加快获得结果，并通过快速实验方案较大限度地使用您的实时 PCR 仪。高浓度制剂使得您能够在运行快速方案所需的较小反应体积中添加更多的靶核酸样品。这使您可以在低滴度研究样品中保持灵敏度，同时提高速度和通量（下图）。

TaqMan Fast 病毒一步法预混液为病毒样品的实时 RT-PCR 提供了一种可靠而有效的试剂，且可维持同等精确度。它在存在常见 RT-PCR 抑制剂的情况下仍具有强大的性能，且其方便灵活的反应构建有助于您获得更可靠的结果。

仅供科研使用。不可用于诊断程序。

规格

适用于（设备）7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System

产品规格管装

热启动内置热启动

Inhibitor Tolerance (Simple)High

多路传输能力Up to 3-plex

反应次数1000 次反应

参比荧光染料ROX（预混）

聚合酶AmpliTaq 快速 DNA 聚合酶

产品线TaqMan

产品类型1步法预混液

数量5 x 1 mL

逆转录酶M-MLV

样品类型总 RNA,病毒 DNA,病毒 RNA,mRNA

运输条件干冰

足够用于1000 次反应

靶标特异性RNA only, Both RNA and DNA

热稳定性Not Applicable

检测方法引物-探针

适用于（应用）基因表达, 病毒检测, DNA Quantitation, Presence/Absence

形式液体

PCR 方法一步法 RT-qPCR

反应速度快速，标准

Unit SizeEach

内容与储存

含有五管 1 mL 管装 4X RT-PCR 预混液,足以进行 1000 次 20-μL 反应。各组分:

•AmpliTaq™ Fast DNA 聚合酶,UP

•耐热 MMLV 逆转录酶

• dNTP

• RNase 抑制剂 (RNaseOUT™)

• ROX™ 染料(惰性参比染料)

管在黑暗环境中于 -20°C 下储存。

常见问题解答 (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

View all TaqMan Fast™ 病毒1步法预混液 FAQs

引用和文献 (959)

引用和文献

Abstract

Diagnosing the novel SARS-CoV-2 by quantitative RT-PCR: variations and opportunities.

Authors:Barreto HG,de Pádua Milagres FA,de Araújo GC,Daúde MM,Benedito VA

Journal:Journal of molecular medicine (Berlin, Germany)

PubMed ID:33067676

The world is currently facing a novel viral pandemic (SARS-CoV-2), and large-scale testing is central to decision-making for the design of effective policies and control strategies to minimize its impact on the global population. However, testing for the presence of the virus is a major bottleneck in tracking the spreading ... More

DNA vaccination protects mice against Zika virus-induced damage to the testes.

Authors:Griffin BD,Muthumani K,Warner BM,Majer A,Hagan M,Audet J,Stein DR,Ranadheera C,Racine T,De La Vega MA,Piret J,Kucas S,Tran KN,Frost KL,De Graff C,Soule G,Scharikow L,Scott J,McTavish G,Smid V,Park YK,Maslow JN,Sardesai NY,Kim JJ,Yao XJ,Bello A,Lindsay R,Boivin G,Booth SA,Kobasa D,Embury-Hyatt C,Safronetz D,Weiner DB,Kobinger GP

Journal:Nature communications

PubMed ID:28589934

Zika virus (ZIKV) is an emerging pathogen causally associated with serious sequelae in fetuses, inducing fetal microcephaly and other neurodevelopment defects. ZIKV is primarily transmitted by mosquitoes, but can persist in human semen and sperm, and sexual transmission has been documented. Moreover, exposure of type-I interferon knockout mice to ZIKV ... More

Inhibitory effect of lactoferrin-coated zinc nanoparticles on SARS-CoV-2 replication and entry along with improvement of lung fibrosis induced in adult male albino rats.

Authors:El-Fakharany EM,El-Gendi H,El-Maradny YA,Abu-Serie MM,Abdel-Wahhab KG,Shabana ME,Ashry M

Journal:International journal of biological macromolecules

PubMed ID:37356684

Severe acute respiratory syndrome 2019-new coronavirus (SARS-CoV-2) is a major global challenge caused by a pandemic disease, named âCOVID-19â with no effective and selective therapy available so far. COVID-19-associated mortality is directly related to the inability to suppress the viral infection and the uncontrolled inflammatory response. So, we investigated the ... More

Respiratory Syncytial Virus Matrix Protein-Chromatin Association Is Key to Transcriptional Inhibition in Infected Cells.

Authors:Li HM,Ghildyal R,Hu M,Tran KC,Starrs LM,Mills J,Teng MN,Jans DA

Journal:Cells

PubMed ID:34685766

The morbidity and mortality caused by the globally prevalent human respiratory pathogen respiratory syncytial virus (RSV) approaches that world-wide of influenza. We previously demonstrated that the RSV matrix (M) protein shuttles, in signal-dependent fashion, between host cell nucleus and cytoplasm, and that this trafficking is central to RSV replication and ... More

Novel strains of a pandemic plant virus, tomato spotted wilt orthotospovirus, increase vector fitness and modulate virus transmission in a resistant host.

Authors:Chinnaiah S,Gautam S,Herron B,Workneh F,Rush CM,Gadhave KR

Journal:Frontiers in microbiology

PubMed ID:37840712

Tomato spotted wilt orthotospovirus (TSWV) is one of the most successful pandemic agricultural pathogens transmitted by several species of thrips in a persistent propagative manner. Current management strategies for TSWV heavily rely on growing single-gene resistant cultivars of tomato (âSw-5bâ gene) and pepper (âTswâ gene) deployed worldwide. However, the emergence ... More

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