Megaplex™ RT 引物，Human Pool Set v3.0
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Applied Biosystems™

Megaplex™ RT 引物，Human Pool Set v3.0

Green features
Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis了解更多信息
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货号数量
444474550 reactions
货号 4444745
价格（CNY)
16,994.00
Each
添加至购物车
数量:
50 reactions
价格（CNY)
16,994.00
Each
添加至购物车
Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® MicroRNA Array. The Human Pool Set v3.0 contains two Megaplex™ RT Primer Pools, Pools A v2.1 and B v3.0. Collectively the pools cover 754 unique microRNAs. Additionally, each pool contains the same 4 controls. The microRNA targets represented in Human Pool A tend be better characterized, more broadly expressed and⁄or expressed at a higher level when compared to the Human Pool B while the microRNA targets represented in Human Pool B tend to be less well characterized, more narrowly expressed and⁄or expressed at a lower level compared to the Human Pool A. The Human Pool Set v3.0 is intended for use with the TaqMan® Array Human MicroRNA Card Set v3.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.
仅供科研使用。不可用于诊断程序。
规格
环保功能绿色可持续包装
反应次数50 次反应
引物RT 引物
产品线Megaplex™
产品类型RT 引物
数量50 reactions
种属人
足够用于50 次反应
适用于（应用）miRNA 分析
高 GC PCR 扩增效果高
PCR 方法qPCR
Unit SizeEach
内容与储存
储存在 -20°C 下。

常见问题解答 (FAQ)

Can Ct's greater than a cut-off be considered valid results?

Yes they can. However, it is important to recognize the true linearity and detection limits of your assay: Ct values above the cut-off can indicate non-specific amplification, unless your NTC is a true- no-target control, and you have run a statistically significant number of replicates. Any results with Ct above the recommended cut-off need to be validated with individual assays on plates.

What is a good Ct cut-off for the TaqMan MicroRNA Array Cards and TaqMan Advanced miRNA Array Cards? In other words, beyond what Ct should I not trust the data?

The typical Ct cut-off on TaqMan Array Cards is 32, which is equivalent to Ct 35 on a plate (10 µl reaction). Previous studies show that if you use pre-amplification, a Ct cut-off of 29 or 30 can be used to reduce numbers of false positives (see Technical Note Optimized protocols for human or rodent microRNA profiling with precious samples). To ensure that you have selected a correct cut-off, you should run replicates of the same sample and use Ct cut-off before you see an increase in the Standard Deviation.

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

View all Megaplex™ RT 引物，Human Pool Set v3.0 FAQs