Megaplex™ 引物池，Human Pools B v3.0
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Applied Biosystems™

Megaplex™ 引物池，Human Pools B v3.0

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Megaplex™ Primer Pools, Human Pools B v3.0 is available under a single part number containing both the Megaplex™ RT Primers了解更多信息
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货号 4444749
价格（CNY)
17,261.00
Each
添加至购物车
价格（CNY)
17,261.00
Each
添加至购物车
Megaplex™ Primer Pools, Human Pools B v3.0 is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Human Pools B v3.0. Megaplex™ RT Primers, Human Pool B v3.0 contains RT primers for 377 unique microRNAs and 4 controls. For use when assay sensitivity of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. The microRNA targets represented in this pool tend be less well characterized, more narrowly expressed and⁄or expressed at a lower level compared to Human Pool A. Megaplex™ Primer Pools, Human Pools B v3.0 are intended for use with the TaqMan® Array Human MicroRNA B Card v3.0, however, they can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.
For Research Use Only. Not for use in diagnostic procedures.
规格
环保功能绿色可持续包装
反应次数50 次反应
引物RT 引物、PreAmp 引物
产品线Megaplex™
产品类型引物池
数量50 reactions
种属人
足够用于50 次反应
适用于（应用）miRNA 分析
高 GC PCR 扩增效果低
PCR 方法qPCR
Unit SizeEach

常见问题解答 (FAQ)

Can Ct's greater than a cut-off be considered valid results?

Yes they can. However, it is important to recognize the true linearity and detection limits of your assay: Ct values above the cut-off can indicate non-specific amplification, unless your NTC is a true- no-target control, and you have run a statistically significant number of replicates. Any results with Ct above the recommended cut-off need to be validated with individual assays on plates.

What is a good Ct cut-off for the TaqMan MicroRNA Array Cards and TaqMan Advanced miRNA Array Cards? In other words, beyond what Ct should I not trust the data?

The typical Ct cut-off on TaqMan Array Cards is 32, which is equivalent to Ct 35 on a plate (10 µl reaction). Previous studies show that if you use pre-amplification, a Ct cut-off of 29 or 30 can be used to reduce numbers of false positives (see Technical Note Optimized protocols for human or rodent microRNA profiling with precious samples). To ensure that you have selected a correct cut-off, you should run replicates of the same sample and use Ct cut-off before you see an increase in the Standard Deviation.