TaqMan™ 阵列人 MicroRNA A+B 卡片套装 v3.0
TaqMan™ 阵列人 MicroRNA A+B 卡片套装 v3.0
Applied Biosystems™

TaqMan™ 阵列人 MicroRNA A+B 卡片套装 v3.0

TaqMan® 阵列人 MicroRNA 卡片套装 v3.0 是一种双卡套装（每张卡总计可进行 384 次 TaqMan®了解更多信息
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货号数量
44449138 pack
货号 4444913
价格（CNY)
31,083.00
Each
添加至购物车
数量:
8 pack
价格（CNY)
31,083.00
Each
添加至购物车
TaqMan® 阵列人 MicroRNA 卡片套装 v3.0 是一种双卡套装（每张卡总计可进行 384 次 TaqMan® MicroRNA 检测）。使用此套装可在短短 5 小时内对 754 个人 microRNA 进行准确定量。每个阵列中包含三种 TaqMan MicroRNA 检测内源参照（可辅助数据标准化）以及一种与人无关的 TaqMan® MicroRNA 检测试剂（作为阴性对照）。有关随附的检测试剂的详细信息见目标列表文件。若运行该阵列集，需要购买 Megaplex™ RT 引物 (Human Pool A v3.0)。当对灵敏度要求较高和/或样本有限时，可使用 Megaplex™ PreAmp 引物（Human Pool 套件 v3.0）进行额外的预扩增步骤。
仅供科研使用。不可用于诊断程序。
规格
适用于（设备）AB 7900 HT 384 孔系统
形式干燥
标签或染料FAM
产品线Megaplex™
数量8 pack
反应速度标准
研究类别基因调控，表观遗传学
类型阵列人 MicroRNA A+B 卡套装
产品规格384孔微流控卡
种属人
Unit SizeEach
内容与储存
4°C

常见问题解答 (FAQ)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.

View all TaqMan™ 阵列人 MicroRNA A+B 卡片套装 v3.0 FAQs