Custom Plus TaqMan™ RNA Assay, VIC
Custom Plus TaqMan™ RNA Assay, VIC
Applied Biosystems™

Custom Plus TaqMan™ RNA Assay, VIC

Applied Biosystems Custom Plus TaqMan RNA assays, VIC, are the custom version of our predesigned TaqMan assays for quantitative real-timeRead more
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Catalog NumberQuantity
4448516L (2900 reactions), made to order
4448515M (750 reactions), made to order
4448514S (360 reactions), made to order
Catalog number 4448516
Price (CNY)
-
Quantity:
L (2900 reactions), made to order

Applied Biosystems Custom Plus TaqMan RNA assays, VIC, are the custom version of our predesigned TaqMan assays for quantitative real-time PCR. Custom Plus TaqMan RNA assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5’ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end.

Features include:
• Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
• Accurate—identify small fold-changes with high accuracy of quantitation
• Custom content—easily design an assay for over 20 species
• Ideal for multiplexing—combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well
• Designed using the full bioinformatic power of the TaqMan Assay design pipeline, which uses proprietary algorithms that have generated millions of assay designs and is regarded as the benchmark in the industry for qPCR design

Approximate ship time
Made to order: 4–6 days in North America, 6–10 days in Europe

Pre-formulated assay
• 2 unlabeled PCR primers (900 nM each final 1X concentration)
• 1 VIC dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Specifications
For Use With (Equipment)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
No. of Reactions2900 reactions
Product LineTaqMan™
QuantityL (2900 reactions), made to order
Shipping ConditionRoom Temperature
Concentration60X
Unit SizeEach
Contents & Storage
1 Tube containing a 20 x (S and M sizes) or 60 x (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store (at -15°C to -25°C.

)

Frequently asked questions (FAQs)

Can I check the end product of a TaqMan Gene Expression Assay by running an agarose gel?

Yes, you can check the end product on an agarose gel. If you use the TaqMan Universal PCR Master Mix (including AmpErase UNG, Cat. No. 4304437), we recommend running the gel immediately after the PCR, since the trace amount of AmpErase UNG may digest the end product that contains double-stranded DNA with dUTP incorporated. The average length of amplicon ranges from 50-150 bp. The exact amplicon length is indicated on the detailed page of each assay on our website. You can get to that page by clicking on the Assay ID; the amplicon information is listed in the section "Assay Details".

How can I reorder a Custom TaqMan Assay that I ordered previously?

To reorder the same Custom TaqMan Assay, you can follow these steps:
- Select an appropriate catalog number, considering the size of the assay that you want. You can find the different catalog numbers for Custom TaqMan Assays on their respective product pages on thermofisher.com.
- Once you have identified the necessary catalog number, go to www.thermofisher.com and click on "Quick Order", in the top right corner of the screen.
- In the following page, paste your selected catalog number in the "Catalog Number" field and paste the assay ID from your previous order in the "Assay ID" field.
- Click "Add to cart" and then in the following screen click "Proceed to check out".

Can I perform a one-step RT-PCR reaction using TaqMan Gene Expression Assays?

While TaqMan Gene Expression Assays can be used to perform a one-step RT-PCR reaction, these assays have not been optimized for this application. TaqMan Gene Expression Assays have been designed, tested, and optimized using Applied Biosystems universal conditions for a two-step RT-PCR reaction.

Did you take into account sequence homology when you developed the TaqMan Gene Expression Assays product?

During the TaqMan Gene Expression Assays product (including TaqMan Gene Expression Assays and Custom Plus TaqMan RNA Assays) design process, we take the following steps to avoid regions of ambiguity: 1. We BLAST the primer and probe designs against transcript databases to ensure specificity to ensure the chosen assay detects only transcript(s) from the gene of interest. 2. We BLAST primer and probe designs against genome databases in order to avoid assays that detect pseudo-genes or genomic DNA.

When preparing the TaqMan or Custom TaqMan Gene Expression Assays, what is the recommended reaction volume?

When preparing the TaqMan or Custom TaqMan Gene Expression Assays, the recommended and supported volume is 20-50 µL for a 96-well standard plate set-up; 10-20 µL for a 96-well fast plate set-up and 5-10 µL for a 384-well set-up. For more information, please consult the TaqMan Gene Expression Assays and Custom TaqMan Gene Expression Assays protocols.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

View all Custom Plus TaqMan™ RNA Assay, VIC, L (2900 reactions), made to order FAQs