Q: 流式细胞仪中的声波辅助流体动力学聚焦的优势体现在哪里？

•模块化设计—多种配置可选，可现场升级。 •节省时间—在不降低数据质量的前提下，速度提高10倍以上。 •简化样品准备流程—无需洗涤，无裂解选项，流体不堵塞。 •可实现独特的应用—可针对广泛的细胞类型和样本构建复杂的实验方案。

Q: Attune NxT声波聚焦流式细胞仪与传统流式细胞仪有何不同？

针对多参数分析应用，Attune NxT声波聚焦流式细胞仪可以选择最多4个激光器和14色配置，采用模块化系统设计来满足大部分试验需求和实验室预算。创新的光路设计，有助于确保四个空间分隔的激光器精确对齐样品流，确保长期的数据一致性、优越的性能和出色的可靠性。仪器可配备多达4个具有平顶光束的固态激光器（405 nm、488 nm、561 nm和637 nm）。 Attune NxT声波聚焦流式细胞仪的声波聚焦技术采用超声波辐射压力（>2 MHz），将微粒运送至样品流中心。之后，这股预聚焦流注入到为样品提供额外流体动力学压力的鞘液流中。这种“声波辅助流体动力学聚焦”技术可以在任何进样速率的情况下都保证样品流中心范围集中、激光照度均一。在传统的依赖于流体动力学聚焦技术的流式细胞仪中，样品中心需要变宽来适应流速的增加，从而导致激光照度不均一。

Q: What are the advantages of acoustic-assisted hydrodynamic focusing in flow cytometry?

-Modular design - Multiple configurations available - field upgradable. -Save time - 10X faster speeds with no loss in data quality. -Simplified sample prep - No wash, no lyse options, non-clogging fluidics. -Enables unique applications - Complex protocols on a broad range of cell types and samples. Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center .

Q: How is the Attune NxT Acoustic Focusing Cytometer different from traditional flow cytometers?

With the option to be configured with up to 4 lasers and 14 colors for multi-parameter analysis the Attune NxT Acoustic Focusing Cytometer was designed as a modular system to fit most experimental needs and lab budgets. The novel design of the optical path helps ensure precise fixed alignment of four spatially separated lasers onto the sample stream enabling consistency in data over time, superior performance, and superior reliability. The instrument can be configured with up to 4 solid-state lasers (405 nm, 488 nm, 561 nm, and 637 nm) with flat top beam profiles. The Attune NxT Flow Cytometer's acoustic focusing uses ultrasonic radiation pressure (> 2 MHz) to transport particles into the center of the sample stream. This pre-focused stream is then injected into the sheath stream, which supplies an additional hydrodynamic pressure to the sample. The combination of these two forces- termed acoustic-assisted hydrodynamic focusing-results in a narrow core stream and uniform laser illumination, regardless of the sample input rate. In traditional cytometers that rely solely on hydrodynamic focusing, the sample core widens to accommodate the increases in flow rate, which results in less uniform laser light illumination. Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center .

Question 1

我能用Attune NxT声波聚焦流式细胞仪检测的大小下限是多少？

Accepted Answer

Attune NxT声波聚焦流式细胞仪检测的下限是0.5 µm。

Question 2

Attune NxT自动进样器有何作用？

Accepted Answer

Attune NxT自动进样器是Attune NxT声波聚焦流式细胞仪的可选附件，能够快速分析至多384份样品。对于不同形式平板——无论96孔还是384孔板——它都具有广泛的兼容性。内置智能探针，最大限度避免堵塞和截留（<0.5%），防止损坏仪器。该仪器通过通气而不是振荡混合，确保样品的均质性，同时维持细胞的活性。它还可作为Attune NxT流式细胞仪关机程序的一个步骤来执行自动清洗。无论采用何种取样方法（试管或平板）和数据采集速率，它都能提供一致的数据。

Question 3

流式细胞仪中的声波辅助流体动力学聚焦的优势体现在哪里？

Accepted Answer

•模块化设计—多种配置可选，可现场升级。
•节省时间—在不降低数据质量的前提下，速度提高10倍以上。
•简化样品准备流程—无需洗涤，无裂解选项，流体不堵塞。
•可实现独特的应用—可针对广泛的细胞类型和样本构建复杂的实验方案。

Question 4

Attune NxT声波聚焦流式细胞仪与传统流式细胞仪有何不同？

Accepted Answer

针对多参数分析应用，Attune NxT声波聚焦流式细胞仪可以选择最多4个激光器和14色配置，采用模块化系统设计来满足大部分试验需求和实验室预算。创新的光路设计，有助于确保四个空间分隔的激光器精确对齐样品流，确保长期的数据一致性、优越的性能和出色的可靠性。仪器可配备多达4个具有平顶光束的固态激光器（405 nm、488 nm、561 nm和637 nm）。

Attune NxT声波聚焦流式细胞仪的声波聚焦技术采用超声波辐射压力（>2 MHz），将微粒运送至样品流中心。之后，这股预聚焦流注入到为样品提供额外流体动力学压力的鞘液流中。这种“声波辅助流体动力学聚焦”技术可以在任何进样速率的情况下都保证样品流中心范围集中、激光照度均一。在传统的依赖于流体动力学聚焦技术的流式细胞仪中，样品中心需要变宽来适应流速的增加，从而导致激光照度不均一。

Question 5

What is the smallest size that I can detect with the Attune NxT Acoustic Focusing Cytometer?

Accepted Answer

The smallest size that you can detect with the Attune NxT Acoustic Focusing Cytometer is 0.5 µm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Question 6

What is the Attune NxT Autosampler?

Accepted Answer

The Attune NxT Autosampler, an optional accessory for the Attune NxT Acoustic Focusing Cytometer, enables rapid processing of up to 384 samples. It has broad compatibility with different plate formats, both 96- and 384-well plates. It has an intelligent probe designed to minimize clogging and carryover (<0.5%) and to prevent damage to the instrument. It mixes by aspiration rather than shaking to ensure homogeneity of the sample and maintain cell viability. Is performs automated cleaning as part of the shutdown process of the Attune NxT Cytometer. It provides consistent data regardless of sampling method (tube vs. plate) and collection rate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Question 7

What are the advantages of acoustic-assisted hydrodynamic focusing in flow cytometry?

Accepted Answer

-Modular design - Multiple configurations available - field upgradable.
-Save time - 10X faster speeds with no loss in data quality.
-Simplified sample prep - No wash, no lyse options, non-clogging fluidics.
-Enables unique applications - Complex protocols on a broad range of cell types and samples.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Question 8

How is the Attune NxT Acoustic Focusing Cytometer different from traditional flow cytometers?

Accepted Answer

With the option to be configured with up to 4 lasers and 14 colors for multi-parameter analysis the Attune NxT Acoustic Focusing Cytometer was designed as a modular system to fit most experimental needs and lab budgets. The novel design of the optical path helps ensure precise fixed alignment of four spatially separated lasers onto the sample stream enabling consistency in data over time, superior performance, and superior reliability. The instrument can be configured with up to 4 solid-state lasers (405 nm, 488 nm, 561 nm, and 637 nm) with flat top beam profiles.

The Attune NxT Flow Cytometer's acoustic focusing uses ultrasonic radiation pressure (> 2 MHz) to transport particles into the center of the sample stream. This pre-focused stream is then injected into the sheath stream, which supplies an additional hydrodynamic pressure to the sample. The combination of these two forces- termed acoustic-assisted hydrodynamic focusing-results in a narrow core stream and uniform laser illumination, regardless of the sample input rate. In traditional cytometers that rely solely on hydrodynamic focusing, the sample core widens to accommodate the increases in flow rate, which results in less uniform laser light illumination.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Attune™ Focusing Fluid (1X), 6 x 1 L - FAQs