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查看更多产品信息 MagMAX™ Express-96 Deep Well Magnetic Particle Processor (PHARMA) - FAQs (4456933)
13 个常见问题解答
您可以在产品页面找到MagMAX mirVana总RNA提取试剂盒在MagMAX Express-96, KingFisher Flex, 或KingFisher Duo仪器上使用的脚本。
KingFisher Flex使用一个USB接口，而MagMAX Express- 96需要一个RS-232接口。
是的，MagMAX Express-96仪器可以和装有最新版本BindIt软件v3.3.1.7的电脑相连。这种连接需要一个到USB转化器的串行端口。已有的.kf2 文件在BindIt软件内不能被编辑，但是可以将.kf2文件重写为.bdz文件从而可以被编辑。请参见应用注意（https://tools.thermofisher.com/content/sfs/brochures/KingFisher-BindIT-TechNote-5189009.pdf）了解更多细节。
不可以，96孔标准磁棒头和96深孔磁棒头不能互换。但是，两台仪器可以使用相同的磁棒套。
The MagMAX beads will cause a loss of ~2-3 µL of solution when they are pulled from the solution, but this is a very small amount. Larger volume loss, such as 20µL, can be due to several factors:
• Low lab humidity combined with a heated elution can cause this amount of liquid to evaporate. In this case, we recommend increasing the starting elution volume. Since the nucleic acid is not evaporating this should not affect your overall yield.
• Issues with alignment of the instrument components could also result in loss of volume. If the head goes down too deep during mixing, it could pull droplets up along the side. This would reduce elution volume and you would also be more likely to see a reduction in yield. A similar issue would result if the turntable alignment was off on either side.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes, the MagMAX Express-96 Deep Well Head can be used on the KingFisher 96 instrument. However, it is not compatible with the KingFisher Flex instrument.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes, the KingFisher Flex instrument 96-well magnetic head and the MagMAX Express-96 instrument magnetic head can use the same tip combs and KingFisher plates even though the magnetic heads are different.
The MagMAX Express-96 protocol scripts can be found on the MagMAX Express protocol page (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/total-rna-extraction/magmax-technology/magmax-particle-processors/magmax-instrument-protocols-scripts.html). If the specific script you are looking for is not located here or on the product page for the MagMAX kit, please contact Technical Support at techsupport@thermofisher.com.
We recommend using the BindIt Software to transfer scripts to the MagMAX Express-96 and KingFisher Flex instruments. The BindIt Software should be used to transfer scripts for all of the Applied Biosystems MagMAX Express and Thermo Scientific KingFisher instruments.
You can find scripts for using MagMAX mirVana Total RNA Isolation Kit with the MagMAX Express-96, KingFisher Flex, or KingFisher Duo instrument, on the product page (http://www.thermofisher.com/order/catalog/product/A27828).
KingFisher Flex uses a USB port, whereas the MagMAX Express- 96 requires a RS-232 port.
Yes, the MagMAX Express-96 instrument is compatible with the BindIt software. A serial port-to-USB converter is required to connect the MagMAX Express-96 instrument to the computer.
If you are using BindIt software v3.3.1.7 you can use it to run existing .kf2 files. You will not be able to edit the .kf2 files in v3.3.1.7.
If you are using BindIt software v4.0 or later, you cannot run or edit existing .kf2 files. To get around this, you can convert the .kf2 files to the BindIt (.bdz) file format and then edit the .bdz file in the BindIt software. Please see the following application note for instructions on how to convert the .kf2 files: (http://www.thermoscientific.com/content/dam/tfs/LPG/LCD/LCD Documents/Application & Technical Notes/Liquid Handling and Dispensing/KingFisher-BindIT-TechNote-5189009.pdf). If you have already converted your .kf2 files to .bdz files, or you plan to convert your .kf2 file to .bdz files, we recommend using BindIt software v4.0 or higher.
No, the 96 well standard and 96 Deep Well heads are not interchangeable between the MagMAX Express-96 and KingFisher Flex instruments. However, the same tip combs can be used for both instruments.