In Vivo Ready 对照品 我们的 In Vivo Ready 对照 siRNA 接受将 siRNA 引入动物所需的纯化和检测。“”经HPLC纯化和退火后,每条siRNA都通过半透膜除去多余的盐分进一步纯化,最终得到含盐量低的高纯度siRNA,适合于体内应用。In vivo siRNA再通过滤过除菌,并已测试了内毒素含量。通过去离子水配制成 50 µM 浓度时,in vivo Ready siRNA 中含有 <5.0 mM Na+、<0.06 mM K+ 和 <0.02 mM Mg2+。
•比非修饰siRNA稳定性>100倍——体内基因敲低效果更持久
•兼容于任何21-mer的序列——敲低能力不会损失
•无毒——实际上降低了siRNA序列特异的免疫反应
•高特异性——脱靶效应最高能降低90%
•提供预设计和经验证两种Ambion In Vivo siRNA
你们提供哪些可用于体内研究的siRNA,它们之间的区别是什么?
请参考下列用于体内研究的不同类型的siRNA:
AmbionIn Vivo预设计siRNA使用Silencer Select算法设计并带有额外化学修饰以获得超强血清稳定性(在37°C下90%小鼠血清中的半衰期>5小时)从而适用于体内实验。 AmbionIn Vivo siRNA在体外(外周血单核细胞;PBMC)和体内(小鼠)均无毒和无免疫源性。在细胞实验中,AmbionIn Vivo siRNA表现出和Silencer Select siRNA相当的效果和特异性。
定制设计的Ambion In VivosiRNA针对您的特定目的基因而设计,使用Silencer Select算法并带有Ambion In Vivo化学修饰。
定制合成的Ambion In VivosiRNA根据您提供的序列合成,带有Ambion In Vivo化学修饰以提高血清稳定性。
我们的AmbionIn Vivo siRNA可以以下列几种纯化级别订购:
脱盐:脱盐并使用MALDI-TOF质谱分析
HPLC:HPLC纯化并使用MALDI-TOF和分析级HPLC分析
In Vivo Ready:HPLC纯化,使用MALDI-TOF和分析级HPLC分析,透析脱盐,过滤灭菌,并经内毒素检测。
What are the benefits of using our Invitrogen In vivo siRNA?
- 100x more stable than unmodified siRNA - longer knockdown in vivo
- Compatible with any 21-mer sequence - no loss of potency
- Non-toxic - actually reduces siRNA sequence specific immune responses
- Enhanced specificity - up to 90% reduction in off target effects
- Both Pre-designed and Validated Invitrogen In Vivo siRNAs offered
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
What siRNA do you offer that I can use for my in vivo studies, and what are the differences between them?
Please see the different siRNA we offer for in vivo studies:
Ambion In Vivo Pre-designed siRNAs are designed using the Silencer Select algorithm and incorporate additional chemical modifications for superior serum stability (half life >5 hours at 37 degrees C in 90% mouse serum) with in vivo applications. Ambion In Vivo siRNAs are non-toxic and non-immunogenic in vitro (peripheral blood nononuclear cells; PBMC) and in vivo (mouse). In cell-based assays, Ambion In Vivo siRNAs exhibit potency and specificity equivalent to Silencer Select siRNAs.
Ambion In Vivo Custom Designed siRNAs are designed to your specified target, using the Silencer Select algorithm and Ambion In Vivo chemical modifications.
Ambion In Vivo Custom Synthesized siRNAs are synthesized with your sequence, with Ambion In Vivo chemical modifications for serum stability.
Our Ambion In Vivo siRNA can be ordered with the following purities:
Desalted: Desalted and analyzed by MALDI-TOF mass spectrometry.
HPLC: HPLC purified and analyzed by MALDI-TOF and analytical HPLC
In Vivo Ready : HPLC purified, analyzed by MALDI-TOF and analytical HPLC, dialyzed to remove salts, sterile filtered, and endotoxin tested
Use our GeneAssist tool to order your custom siRNA (http://www.thermofisher.com/order/custom-genomic-products/tools/sirna/).
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.