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查看更多产品信息 Custom Designed Ambion™ In Vivo - FAQs (4457296)
4 个常见问题解答
•比非修饰siRNA稳定性>100倍——体内基因敲低效果更持久
•兼容于任何21-mer的序列——敲低能力不会损失
•无毒——实际上降低了siRNA序列特异的免疫反应
•高特异性——脱靶效应最高能降低90%
•提供预设计和经验证两种Ambion In Vivo siRNA
请参考下列用于体内研究的不同类型的siRNA：
AmbionIn Vivo预设计siRNA使用Silencer Select算法设计并带有额外化学修饰以获得超强血清稳定性（在37°C下90%小鼠血清中的半衰期>5小时）从而适用于体内实验。 AmbionIn Vivo siRNA在体外（外周血单核细胞；PBMC）和体内（小鼠）均无毒和无免疫源性。在细胞实验中，AmbionIn Vivo siRNA表现出和Silencer Select siRNA相当的效果和特异性。
定制设计的Ambion In VivosiRNA针对您的特定目的基因而设计，使用Silencer Select算法并带有Ambion In Vivo化学修饰。
定制合成的Ambion In VivosiRNA根据您提供的序列合成，带有Ambion In Vivo化学修饰以提高血清稳定性。
我们的AmbionIn Vivo siRNA可以以下列几种纯化级别订购：
脱盐：脱盐并使用MALDI-TOF质谱分析
HPLC：HPLC纯化并使用MALDI-TOF和分析级HPLC分析
In Vivo Ready：HPLC纯化，使用MALDI-TOF和分析级HPLC分析，透析脱盐，过滤灭菌，并经内毒素检测。
使用我们的GeneAssist工具（https://www.thermofisher.com/order/custom-genomic-products/tools/sirna/）订购您的定制siRNA。
- 100x more stable than unmodified siRNA - longer knockdown in vivo
- Compatible with any 21-mer sequence - no loss of potency
- Non-toxic - actually reduces siRNA sequence specific immune responses
- Enhanced specificity - up to 90% reduction in off target effects
- Both Pre-designed and Validated Invitrogen In Vivo siRNAs offered
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
Please see the different siRNA we offer for in vivo studies:
Ambion In Vivo Pre-designed siRNAs are designed using the Silencer Select algorithm and incorporate additional chemical modifications for superior serum stability (half life >5 hours at 37 degrees C in 90% mouse serum) with in vivo applications. Ambion In Vivo siRNAs are non-toxic and non-immunogenic in vitro (peripheral blood nononuclear cells; PBMC) and in vivo (mouse). In cell-based assays, Ambion In Vivo siRNAs exhibit potency and specificity equivalent to Silencer Select siRNAs.
Ambion In Vivo Custom Designed siRNAs are designed to your specified target, using the Silencer Select algorithm and Ambion In Vivo chemical modifications.
Ambion In Vivo Custom Synthesized siRNAs are synthesized with your sequence, with Ambion In Vivo chemical modifications for serum stability.
Our Ambion In Vivo siRNA can be ordered with the following purities:
Desalted: Desalted and analyzed by MALDI-TOF mass spectrometry.
HPLC: HPLC purified and analyzed by MALDI-TOF and analytical HPLC
In Vivo Ready : HPLC purified, analyzed by MALDI-TOF and analytical HPLC, dialyzed to remove salts, sterile filtered, and endotoxin tested
Use our GeneAssist tool to order your custom siRNA (http://www.thermofisher.com/order/custom-genomic-products/tools/sirna/).
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.