Single Cell Lysis Kit - FAQs

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2 个常见问题解答

What is the minimum amount of cells required to perform RNA isolation using the Single Cell Lysis Kit?

The Single Cell Lysis Kit enables RNA preparation from 1-10 cells. It generates a concentrated cell lysate through the optimized combination of lysis buffer, stop solution, and DNase. The resulting lysate is compatible with common enzymatic reactions such as reverse transcription. Sample preparation is fast and can typically be completed in around 7 minutes with 2 pipetting steps.

Can laser micro-dissected FFPE single cells be used with a Single Cell-to-CT Kit?

While we have not tried this in-house, there is reason to believe this should work. However, optimization of the protocol around the RT step may be needed, as FFPE samples have a lot of RNA crosslinking, which could affect the efficiency of the RT enzyme. Heating the sample for 10 min at 80 degrees C before adding the RT in order to get good cDNA conversion should improve reverse transcription.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.