TaqMan™ 多重预混液含有 AmpliTaq™ Fast DNA 聚合酶、尿嘧啶-N-糖基化酶 (UNG)、dNTP(包含 dUTP)、MUSTANG PURPLE™ 染料(惰性参比)和优化的缓冲液。
冷藏储存 (2–8°C)。
常见问题解答 (FAQ)
I need to multiplex but cannot use ROX. What options do I have?
For multiplex assays that include reporter dyes overlapping the ROX channel (i.e. JUN), we recommend using one of our optimized multiplex master mixes that include the passive reference dye, MUSTANG PURPLE. MUSTANG PURPLE is compatible with QuantStudio 5, 6, 7, and 12K Flex, 7500, 7500 Fast, and ViiA 7 real-time PCR instruments.
While not ideal, we also offer master mixes that contain no passive reference dyes. For selection help for master mixes with no passive reference, please contact Technical Support at techsupport@thermofisher.com.
What master mix should I use for multiplexing in my qPCR experiments?
The choice of master mix depends upon the number of assays in your reaction, the application, and instrument compatibility. Please refer to our real-time PCR master mixes and instrument compatibility chart (https://assets.thermofisher.com/TFS-Assets/LSG/brochures/master-mix-instrument-compatibility.pdf) prior to selecting one of the master mixes below:
Gene Expression Assays:
- Single, duplex, and triplex assays: TaqMan Fast Advanced Master Mix or TaqMan Fast Virus 1-Step Master Mix
- Multiplexing up to 4 assays: TaqMan Multiplex Master Mix or TaqPath 1-Step Multiplex Master Mix
SNP Genotyping Assays:
- Single SNP Genotyping assay: TaqPath ProAmp Master Mix
- Duplex SNP Genotyping assays: TaqPath ProAmp Multiplex Master Mix, TaqMan Multiplex Master Mix