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Invitrogen

Phospho-AKT1 (Ser473) Monoclonal Antibody (14-6)

Advanced Verification
34 Published Figures
60 References
View all (149) AKT1 antibodies

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Phospho-AKT1 (Ser473) Monoclonal Antibody (14-6)

Product Details

44-621G

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 57 publications 57 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Proximity Ligation Assay (PLA) (PLA)

-
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse

Published species

Chicken, Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

14-6

Immunogen

The antibody was produced against a chemically synthesized phosphopeptide derived from the region of human Akt that contains serine 473.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA, 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533699

Product Specific Information

This product contains enough material for 10 mini-blots. The immunogenic sequence is conserved among multiple species including human, mouse, and rat in all three isoforms: Akt1, Akt2, and Akt3. Others species (100% homologous) have not been tested. Recommended positive controls include NIH-3T3 cells stimulated with PDGF and 3T3-L1 adipocytes stimulated with OSM and IFN-gamma.

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; C-AKT; CAKT; PKB; PKB alpha; PKB beta; PKB gamma; PKBG; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; unnamed protein product; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1

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Gene Aliases: AKT; AKT1; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA

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UniProt ID: (Human) P31749, (Mouse) P31750

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Entrez Gene ID: (Human) 207, (Mouse) 11651

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding calmodulin binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity transferase activity enzyme binding kinase binding protein kinase binding nitric-oxide synthase regulator activity protein serine/threonine kinase inhibitor activity identical protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding 14-3-3 protein binding potassium channel activator activity protein serine kinase activity TORC2 complex binding protein kinase C binding transferase activity, transferring phosphorus-containing groups protein phosphatase 2A binding non-receptor serine/threonine protein kinase
Process(es)
osteoblast differentiation maternal placenta development positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis cytoplasmic translation complement receptor mediated signaling pathway sphingosine-1-phosphate signaling pathway glycogen metabolic process glycogen biosynthetic process regulation of glycogen biosynthetic process glucose metabolic process gluconeogenesis translational initiation regulation of translation protein phosphorylation ubiquitin-dependent protein catabolic process protein import into nucleus NLS-bearing protein import into nucleus nitric oxide biosynthetic process ER to Golgi vesicle-mediated transport Golgi to plasma membrane transport apoptotic process activation-induced cell death of T cells inflammatory response response to oxidative stress signal transduction epidermal growth factor receptor signaling pathway G-protein coupled receptor signaling pathway germ cell development nervous system development cell proliferation insulin receptor signaling pathway apoptotic mitochondrial changes cellular response to starvation response to heat response to hormone gene expression negative regulation of autophagy positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport fibroblast migration positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of glucose metabolic process regulation of neuron projection development negative regulation of macroautophagy phosphorylation cell migration protein ubiquitination peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cytokine-mediated signaling pathway oogenesis stage cell differentiation protein catabolic process positive regulation of cell growth regulation of cell migration positive regulation of cell migration negative regulation of cell migration anaphase-promoting complex-dependent catabolic process SCF-dependent proteasomal ubiquitin-dependent protein catabolic process T cell costimulation negative regulation of protein ubiquitination regulation of myelination lipopolysaccharide-mediated signaling pathway cellular response to nutrient levels TOR signaling negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity response to food peripheral nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus regulation of protein localization positive regulation of peptidyl-serine phosphorylation cellular response to stress negative regulation of toll-like receptor signaling pathway positive regulation of toll-like receptor signaling pathway toll-like receptor 4 signaling pathway cellular response to amino acid starvation response to fluid shear stress protein K29-linked ubiquitination intracellular signal transduction interleukin-18-mediated signaling pathway cellular response to vascular endothelial growth factor stimulus cellular response to decreased oxygen levels SREBP-SCAP complex retention in endoplasmic reticulum tRNA stabilization calcium ion transmembrane import into mitochondrion PERK-mediated unfolded protein response TORC1 signaling glucose homeostasis regulation of apoptotic process Golgi to plasma membrane protein transport negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process anoikis regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of cholesterol biosynthetic process positive regulation of fat cell differentiation positive regulation of cilium assembly positive regulation of glycogen biosynthetic process negative regulation of Notch signaling pathway negative regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter negative regulation of translational initiation positive regulation of translational initiation nitric oxide metabolic process positive regulation of glucose import positive regulation of organ growth protein autophosphorylation positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway behavioral response to pain positive regulation of smooth muscle cell proliferation striated muscle cell differentiation positive regulation of protein metabolic process protein maturation excitatory postsynaptic potential response to growth hormone regulation of vesicle-mediated transport mammary gland epithelial cell differentiation labyrinthine layer blood vessel development protein localization to cilium response to UV-A response to growth factor protein localization to endoplasmic reticulum protein K11-linked ubiquitination cellular response to calcium ion cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus tricarboxylic acid metabolic process establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion cellular response to rapamycin protein K6-linked ubiquitination negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis vascular endothelial cell response to laminar fluid shear stress regulation of postsynapse organization tRNA (guanine-N7)-methylation regulation of tRNA methylation cellular response to oxidised low-density lipoprotein particle stimulus cGAS/STING signaling pathway negative regulation of protein localization to lysosome negative regulation of cGAS/STING signaling pathway beta-arrestin-dependent dopamine receptor signaling pathway positive regulation of G1/S transition of mitotic cell cycle positive regulation of protein localization to nucleus cellular response to peptide regulation of signal transduction by p53 class mediator negative regulation of cilium assembly negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of leukocyte cell-cell adhesion positive regulation of protein localization to plasma membrane negative regulation of protein maturation negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway negative regulation of PERK-mediated unfolded protein response negative regulation of TORC1 signaling positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of protein localization to endoplasmic reticulum positive regulation of anaphase-promoting complex-dependent catabolic process cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus positive regulation of protein localization to cell surface regulation of type B pancreatic cell development negative regulation of lymphocyte migration negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway protein import into nucleus, translocation positive regulation of protein phosphorylation carbohydrate metabolic process translation negative regulation of protein kinase activity transport cytoskeleton organization multicellular organism development aging carbohydrate transport negative regulation of endopeptidase activity glucose transport cell projection organization positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle positive regulation of cellular protein metabolic process anagen positive regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of cell size positive regulation of vasoconstriction negative regulation of JNK cascade positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity glycogen cell differentiation involved in embryonic placenta development cellular response to mechanical stimulus cellular response to organic cyclic compound cellular response to hypoxia positive regulation of establishment of protein localization to plasma membrane
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