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Invitrogen

Phospho-FAK (Tyr861) Polyclonal Antibody

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70 篇参考文献
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Phospho-FAK (Tyr861) Polyclonal Antibody

产品信息

44-626G

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000
查看36篇已发表文章 36篇已发表文章

免疫组化 (IHC)

Assay-dependent
查看13篇已发表文章 13篇已发表文章

免疫组化（石蜡） (IHC (P))

-
查看5篇已发表文章 5篇已发表文章

免疫细胞化学 (ICC/IF)

1:250
查看13篇已发表文章 13篇已发表文章

免疫沉淀 (IP)

-
查看2篇已发表文章 2篇已发表文章

其他PubMed (Misc)

-
查看1篇已发表文章 1篇已发表文章
产品规格

种属反应

Chicken, Human, Mouse

已发表种属

Chicken, Dog, Hamster, Human, Mouse, Non-human primate, Rat, Xenopus, Zebrafish

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 861. The sequence is conserved in mouse, rat, chicken and frog.
3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533703

产品详细信息

The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated FAK. The final product is generated by affinity chromatography using a FAK-derived peptide that is phosphorylated at tyrosine 861.

Primary chicken embryo fibroblasts (CEF) expressing FAK protein and plated on fibronectin, or A459 whole cell lysate was used as positive control.

The target antigen, in western blotting, has an apparent MW of 125 kDa.

靶标信息

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: EC 2.7.10.2; FADK 1; FAK-Del33; exon 33 deletion splicing mutant; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; p41/p43FRNK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; tyrosine kinase; unnamed protein product

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基因别名: FADK; FADK 1; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2

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UniProt ID: (Human) Q05397, (Mouse) P34152

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Entrez Gene ID: (Human) 5747, (Chicken) 396416, (Mouse) 14083

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功能
nucleotide binding actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein tyrosine phosphatase activity integrin binding protein binding ATP binding JUN kinase binding kinase activity transferase activity protein kinase binding protein phosphatase binding SH2 domain binding molecular function activator activity protease binding calcium-dependent cysteine-type endopeptidase activity receptor binding identical protein binding signal transducer activity transferase activity, transferring phosphorus-containing groups protein complex binding phosphatidylinositol 3-kinase binding
参与通路
MAPK cascade microtubule cytoskeleton organization angiogenesis blood vessel development vasculogenesis neuron migration regulation of protein phosphorylation positive regulation of protein phosphorylation protein phosphorylation movement of cell or subcellular component cell adhesion signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway JNK cascade multicellular organism development positive regulation of cell proliferation regulation of cell shape regulation of epithelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration phosphorylation peptidyl-tyrosine phosphorylation central nervous system neuron axonogenesis negative regulation of cell-cell adhesion regulation of cell adhesion extracellular matrix organization positive regulation of cell growth positive regulation of cell migration negative regulation of cell migration cellular chloride ion homeostasis regulation of cell adhesion mediated by integrin peptidyl-tyrosine autophosphorylation establishment of nucleus localization regulation of cell proliferation negative regulation of apoptotic process endothelial cell migration innate immune response regulation of osteoblast differentiation positive regulation of cell adhesion positive regulation of protein kinase activity positive regulation of vasodilation negative regulation of organ growth protein autophosphorylation ephrin receptor signaling pathway positive regulation of smooth muscle cell proliferation positive regulation of phagocytosis negative regulation of axonogenesis positive regulation of synaptic transmission regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of synapse assembly positive regulation of glial cell proliferation growth hormone receptor signaling pathway cellular response to transforming growth factor beta stimulus regulation of substrate adhesion-dependent cell spreading positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of anoikis placenta development regulation of cell-matrix adhesion heart morphogenesis axon guidance regulation of endothelial cell migration positive regulation of epithelial cell migration positive regulation of epithelial to mesenchymal transition positive regulation of macrophage chemotaxis positive regulation of fibroblast migration cell migration establishment of cell polarity cell differentiation detection of muscle stretch netrin-activated signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis regulation of GTPase activity regulation of cell differentiation vascular endothelial growth factor receptor signaling pathway cell motility regulation of multicellular organismal process regulation of cytoskeleton organization positive regulation of wound healing vascular endothelial cell response to oscillatory fluid shear stress positive regulation of macrophage proliferation proteolysis actin filament organization transmembrane receptor protein tyrosine kinase signaling pathway response to pH negative regulation of cell-substrate adhesion pyramidal neuron migration negative regulation of protein autophosphorylation positive regulation of protein binding response to muscle stretch wound healing, spreading of cells positive regulation of focal adhesion assembly angiogenesis involved in wound healing positive regulation of protein tyrosine kinase activity positive regulation of substrate-dependent cell migration, cell attachment to substrate
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