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Platinum™ Multiplex PCR Master Mix, 250 reactions - FAQs

查看更多产品信息 Platinum™ Multiplex PCR Master Mix - FAQs (4464269, 4464268, 4464270)

4 个常见问题解答

Platinum 技术 和 AccuPrime 技术有何差异？

使用Platinum 技术时，在94°C的变性步骤之前，抗DNA聚合酶的抗体结合在酶上。在变性步骤中，抗体将随之降解，聚合酶活化，引物开始介导延伸。AccuPrime Taq将PlatinumTaq (Taq + Platinum抗体) 与我们专利的热稳定 AccuPrime 辅助蛋白相结合。10x反应缓冲液中包含了该辅助蛋白，以提高每轮PCR反应中引物和模板结合的特异性。

Does the Platinum Multiplex PCR master mix leave A overhangs?

Yes, the Platinum Multiplex PCR master mix contains Platinum Taq DNA Polymerase that leaves A overhangs on PCR products.

What is the difference between Platinum technology and AccuPrime technology?

With Platinum technology, anti-DNA polymerase antibodies bind to the enzyme until the denaturing step at 94 degrees C, when the antibodies degrade. The polymerase is now active and primer extension can occur. AccuPrime Taq combines Platinum Taq (Taq + Platinum antibodies) with proprietary thermostable AccuPrime accessory proteins. The 10X reaction buffer contains the accessory proteins which enhance specific primer-template hybridization during each cycle of PCR.

Can I use Platinum Multiplex PCR Master Mix for amplifying uracil-containing templates?

Yes, Platinum Multiplex PCR Master Mix contains Platinum Taq Polymerase that can incorporate dUTP and amplify uracil-containing templates.