Search
Search
查看更多产品信息 E-Gel™ Imager UV/SYBR™ Filter - FAQs (4466608)
21 个常见问题解答
1) 检查相机罩或激活键处于正确位置以确保基座是激活的。
2) 光基座可能“超时”，但是电源指示灯仍然是亮的。关闭基座，然后重新打开。
按下激活按钮超过2秒之后，成像仪投射发光的时间将变成5分钟而不是30秒。
这一错误出现是因为有其它软件在后台运行，例如Internet Explorer浏览器，或者有杀毒软件正在运行从而限制了对一些文件的访问。
要解决这一问题，可以试试：
•在安装过程中关闭Internet Explorer浏览器或任何其它程序。
•关闭杀毒程序（或暂时卸载它），安装E-Gel 成像仪软件，然后重新激活或安装杀毒软件。
•当错误出现时，选择“Ignore”以允许安装继续进行而不是“Retry（重试）”或“Abort（放弃）”。
我们目前不提供和Mac电脑兼容的软件。请在装有 Windows XP Professional或Windows 7 Professional操作系统的32-bit或64-bit PC上运行该软件。
对于E-Gel成像仪，我们推荐使用紫外光基座和E-Gel成像仪通用滤光片观察EB凝胶，使用蓝光基座和E-Gel成像仪通用滤光片或紫外光基座和E-Gel成像仪UV/SYBR 滤光片（绿色滤片）观察SYBR凝胶。
两种软件都同时随E-Gel凝胶成像系统系统提供。GelCapture软件用于在成像过程中对相机罩进行控制并在拍照后对图像进行基本操作。GelQuant Express软件用于在成像结束后对图像进行分析。这包括对目的条带的大小和相对或绝对亮度进行估计，以及在有需要时生成一份整张凝胶的数据报告。GelQuant Express软件仅能在密码狗（HASP密钥）插入电脑的USB插口上时才能使用。
以下是该系统的技术指标：
•连接：USB2.0
•相机电源：AC: 100–240V, 50–60 Hz; DC: 7.5 V 2.0 A
•相机罩尺寸：35.6 cm (高) × 28.4 cm (长) × 20.3 cm (宽)
•电力要求：100–240 V, 50/60 Hz, 0.6 A
•温度：室温± 5°C到40°C
•基座尺寸：11.9 cm (高) × 30.4 cm (长) × 21.4 cm (宽) (此处是指整个基座)
•观察表面尺寸：15 cm x 12 cm (此处是指放置凝胶的区域)
•适配器技术指标：只能使用与E-Gel 成像仪相机罩(100–240 VAC, 50/60 Hz, 0.6 A)同时提供的UL清单中的适配器。
这些E-Gel成像系统滤光片是可替换滤光片，您只需取出通用滤光片盘并插入自己选择的滤光片盘就可方便的使用。我们提供一种橙色E-Gel成像系统通用滤光片，一种适用于SYBR Green染料和SYBR Safe染料以及E-Gel EX琼脂糖凝胶所用染料的绿色滤光片，以及一种适用于我们的Molecular Probes Qdot 625产品的红色滤光片。
E-Gel成像系统有三种基座可选：
1. 蓝光透射基座——非常适用于新型DNA染料，如SYBR Safe染料，SYBR Green染料，以及E-Gel EX琼脂糖凝胶中使用的染料。
2. 紫外透射基座——最适于传统的溴化乙啶染色。
3. E-Gel适配器基座——一种与E-Gel Go!系统或者E-Gel iBase/E-Gel Safe Imager系统兼容的实时凝胶记录基座。
请点击此链接查看E-Gel 成像系统的参数信息。
1) Check that the camera hood or activator key is in place to make sure the base is not deactivated.
2) Light bases will "time out," but the power indicator light may stay illuminated. Turn the base off, then back on.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
Depress the activation button for at least 2 seconds, and the imager will transilluminate for 5 minutes rather than 30 seconds.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
This error can occur if there is other software running in the background, like an Internet Explorer browser, or if there is an antivirus program running that is limiting his ability to access some files.
To fix this error, try to:
- Turn off Internet Explorer or any other program running during the install.
- Inactivate the antivirus program (or temporarily uninstall it), install the E-Gel Imager software, then reactivate or reinstall the antivirus program.
- When the error comes up, select "Ignore" to allow the installation to continue instead of "Retry" or "Abort".
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
We do not currently offer software that is compatible with Mac computers. Please install the software on a 32-bit or 64-bit PC running either a Windows XP Professional or Windows 7 Professional operating system.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
For our E-Gel Imager, we recommend using the UV light base with the E-Gel Imager Universal Filter for ethidium bromide gels, and either the blue light base with the E-Gel Imager Universal Filter or the UV light base with E-Gel Imager UV/SYBR Filter (green filter) for SYBR gels.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
Both types of software come with each E-Gel Imager system. GelCapture software is used to control the camera hood during image acquisition and to perform basic manipulations to the image once acquired. The GelQuant Express software is used to analyze images after they have been acquired. This includes estimating the size and relative or absolute mass of bands of interest as well as providing a data report for an entire gel if needed. GelQuant Express software can only be used on a given computer while the activation dongle (HASP key) is inserted into a USB port.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
Here are the specifications:
- Connectivity: USB2.0
- Camera power supply: AC: 100-240V, 50-60 Hz; DC: 7.5 V 2.0 A
- Camera hood dimensions: 35.6 cm (height) x 28.4 cm (length) x 20.3 cm (width)
- Electrical requirements: 100-240 V, 50/60 Hz, 0.6 A
- Temperature: Ambient ±5°C to 40°C
- Base dimensions: 11.9 cm (height) x 30.4 cm (length) x 21.4 cm (width) (this is the entire base)
- Viewing surface dimensions: 15 cm x 12 cm (this is the area where the gel is placed)
- Adaptor specifications: Use only the UL Listed adaptor supplied with the E-Gel Imager Camera Hood (100-240 VAC, 50/60 Hz, 0.6 A)
- Weight: 1 kg
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
These E-Gel Imager Filters are alternative filters that can be easily utilized by removing the universal filter tray and sliding in the filter tray of your choosing. We offer an orange E-Gel Imager Universal Filter, a green filter optimal for SYBR Green and SYBR Safe stains and those found in E-Gel EX Agarose Gels, and a red filter for use with our Invitrogen Qdot 625 products.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
Yes, you will need to purchase the E-Gel Imager White-Light Conversion Screen (Cat. No. 4473061), that converts blue light emitted by the blue-light transilluminator or UV light emitted by the UV-light transilluminator to white light. This conversion screen is compatible with multiple protein stains including SimplyBlue SafeStain, SilverQuest silver stain, and Coomassie blue stains.
Gels stained with SYPRO Ruby Protein Gel Stain do not need the white-light conversion screen. They can be visualized using a E-Gel Imager Qdot 625 Filter (Cat. No. 4466607).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
There are three base options for the E-Gel Imager:
1.Blue-light transilluminator base-ideal for innovative DNA stains such as SYBR Safestains, SYBR Green stains, and those found in E-Gel EX Agarose Gels.
2.UV transilluminator base-best for gels traditionally stained with ethidium bromide.
3.E-Gel adapter base-a one-of-a-kind, real-time gel documentation base that is compatible with the E-Gel Go! System or E-Gel iBase/E-Gel Safe Imager System.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
Please use this link (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/e-gel-electrophoresis-system/e-gel-imager-system.html#specs) to view the E-Gel Imager specifications.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.