Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 微型制备试剂盒
Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 微型制备试剂盒
Thermo Scientific™

Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 微型制备试剂盒

The Thermo Scientific Pierce Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit uses immobilized ficin protease to efficiently digest mouse了解更多信息
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货号数量
4468010份抗体样品,每份含 25 至 250µg IgG
货号 44680
价格(CNY)
8,456.00
Each
添加至购物车
数量:
10份抗体样品,每份含 25 至 250µg IgG
请求批量或定制报价
价格(CNY)
8,456.00
Each
添加至购物车
The Thermo Scientific Pierce Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit uses immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.

Features of the Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit:

Capacity—kit provides for fragmenting 25 to 250 μg IgG.
Complete—kit includes all reagents needed to prepare and purify antibody fragments
Enzyme-free digestion products—Immobilized Ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme
Optimized for mouse IgG1—the kit procedure is optimized for mouse IgG1; the only effective method for this IgG species and subclass (see

Related Products
Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kit
Pierce™ F(ab')2 Micro Preparation Kit
Pierce™ Fab Micro Preparation Kit
仅供科研使用。不可用于诊断程序。
规格
数量10份抗体样品,每份含 25 至 250µg IgG
产品线Pierce™
Unit SizeEach
内容与储存
足够用于:10 个抗体样品、每个样品含有 25 至 250 µg IgG
• Pierce 无花果蛋白酶琼脂糖,0.8 mL
• 半胱氨酸-HCl,1 g
• 小鼠 IgG1 酶切缓冲液,120 mL
• NAb 蛋白 A Plus 离心柱,0.2 mL,2 柱
• 蛋白 A 结合缓冲液,50 mL
• IgG 洗脱缓冲液,50 mL
• Zeba 脱盐离心柱,7K MWCO,0.5 mL,10 柱
• 离心柱,0.8 mL,10 柱
• 微量离心管,2 mL,30 支

在 4–8°C 下储存。

常见问题解答 (FAQ)

Can I reuse the immobilized digestion enzymes in the Thermo Scientific IgG Fragmentation kit?

No. For best results, use the immobilized enzyme once and discard after use.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long does IgG digestion take using the Thermo Scientific IgG Fragmentation kit?

The digestion time is a function of the IgG concentration and source. For rabbit, mouse and human IgG, optimized digestion times are given in the instructions for each kit. Digestion times listed in the product instructions result in 80-90% digestion, although efficiency varies depending on IgG preparation and source.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

In the Thermo Scientific IgG Fragmentation kit, when digesting an IgG that does not bind to Protein A, how do I purify the Fab and F(abÁ)2 fragments?

If the IgG is a species or subclass that does not bind to Protein A, researchers can immobilize an antibody specific for the desired fragment. For example, to purify digested rat IgG2a, use the Aminolink Plus Immobilization Kit (Cat. No. 44894) to immobilize goat anti-rat Fc antibody. Our Tech Tip #34 and other web resources list IgG binding properties of Protein A. (Because Protein G and Protein A/G bind Fab fragments, they are not effective for this application.)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I purchase the immobilized enzymes (proteases) from the Thermo Scientific IgG Fragmentation kit separately?

Yes. The three immobilized enzymes are available in 5 mL package sizes as shown below:

• Immobilized Ficin, Cat. No. 44881
• Immobilized Papain, Cat. No. 20341
• Immobilized Pepsin, Cat. No. 20343

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many Thermo Scientific IgG Fragmentation kits are available and which enzymes are included?

There are six Thermo Scientific IgG fragmentation kits:

• Fab Preparation Kit (Papain), Cat. No. 44985
• Fab Micro Preparation Kit (Papain), Cat. No. 44685
• F(abÁ)2 Preparation Kit (Pepsin), Cat. No. 44988
• F(abÁ)2 Micro Preparation Kit, (Pepsin), Cat. No. 44688
• Mouse IgG1 Fab and F(abÁ)2 Preparation Kit (Ficin), Cat. No. 44980
• Mouse IgG1 Fab and F(abÁ)2 Micro Preparation Kit (Ficin), Cat. No. 44680

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.