Pierce™ F(ab')2 微型制备试剂盒
Pierce™ F(ab')2 微型制备试剂盒
Pierce™ F(ab')2 微型制备试剂盒
Pierce™ F(ab')2 微型制备试剂盒
Thermo Scientific™

Pierce™ F(ab')2 微型制备试剂盒

使用固定化胃蛋白酶选择性酶切全人和其他 IgG 抗体,以制成保留抗原结合活性的 F(ab')2 片段。
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货号数量
4468810份抗体样品,每份含 25 至 250μg IgG
货号 44688
价格(CNY)
7,382.00
Each
数量:
10份抗体样品,每份含 25 至 250μg IgG
请求批量或定制报价
价格(CNY)
7,382.00
Each

Thermo Scientific™ Pierce F(ab')2 制备试剂盒使用固定化胃蛋白酶选择性酶切全人和其他 IgG 抗体,以制成保留抗原结合活性的 F(ab')2 片段。

这些试剂盒使用胃蛋白酶(一种非特异性肽链内切酶)对全 IgG 的 Fc 部分进行酶促酶切,得到称为 F(ab')2 的片段。该片段由一对通过两个二硫键连接的 Fab' 单元构成。由于胃蛋白酶提供为固定化的球珠状琼脂糖树脂形式,消化反应可以通过从 IgG 溶液中去除树脂而轻易地终止,获得无酶的消化产品。优化的程序、高性能脱盐以及蛋白 A 离心柱使这些试剂盒易于使用。

特点:

  • 无酶的酶切产品 – 固定化胃蛋白酶(微珠状琼脂糖树脂)可用于控制酶切反应,并完全去除蛋白酶中的所得抗体片段

  • 适用于人和其他物种的 IgG – 该试剂盒程序针对人、兔和小鼠 IgG 进行了优化,但基于胃蛋白酶的酶切对于其他物种和亚类的 IgG 有效(注:对于小鼠 IgG1,为获得较佳结果,请使用产品编号44980)

  • 高容量 – 该试剂盒可一次使用多达 4mg IgG 进行酶切和纯化。使用标准大小试剂盒可对 0.25 至 4mg IgG 进行片段化,微型试剂盒可对 25 至 250μg IgG 进行片段化

  • 完整且易于使用 – 试剂盒包括制备和纯化抗体片段所需的所有试剂、离心柱和附件

包括:

琼脂糖树脂胃蛋白酶、消化缓冲液、蛋白 A 离心柱(带结合和洗脱缓冲液)、脱盐离心柱及配件

仅供科研使用。不可用于诊断程序。
规格
套装内容Pierce F(ab')2 微型制备试剂盒
数量10份抗体样品,每份含 25 至 250μg IgG
靶标基因ERK5/BMK1 (pan)
产品线Pierce
Unit SizeEach
内容与储存
足够用于:10 份抗体样品,每份含 25 至 250 μg IgG
• Pierce 胃蛋白酶琼脂糖,0.5 mL
•,F(ab')2 酶切缓冲液,55 mL
• NAb 蛋白 A Plus 离心柱,0.2 mL,2 根柱
• PBS 包(每个可制备 500 mL),2 包
• IgG 洗脱缓冲液,50 mL
• Zeba 脱盐离心柱,7K MWCO,0.5 mL,10 根柱
• 离心柱,0.8 mL,10 根柱
• 微量离心管,2 mL,30 个管

储存在 4°C 下

常见问题解答 (FAQ)

Can I reuse the immobilized digestion enzymes in the Thermo Scientific IgG Fragmentation kit?

No. For best results, use the immobilized enzyme once and discard after use.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long does IgG digestion take using the Thermo Scientific IgG Fragmentation kit?

The digestion time is a function of the IgG concentration and source. For rabbit, mouse and human IgG, optimized digestion times are given in the instructions for each kit. Digestion times listed in the product instructions result in 80-90% digestion, although efficiency varies depending on IgG preparation and source.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

In the Thermo Scientific IgG Fragmentation kit, when digesting an IgG that does not bind to Protein A, how do I purify the Fab and F(abÁ)2 fragments?

If the IgG is a species or subclass that does not bind to Protein A, researchers can immobilize an antibody specific for the desired fragment. For example, to purify digested rat IgG2a, use the Aminolink Plus Immobilization Kit (Cat. No. 44894) to immobilize goat anti-rat Fc antibody. Our Tech Tip #34 and other web resources list IgG binding properties of Protein A. (Because Protein G and Protein A/G bind Fab fragments, they are not effective for this application.)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I purchase the immobilized enzymes (proteases) from the Thermo Scientific IgG Fragmentation kit separately?

Yes. The three immobilized enzymes are available in 5 mL package sizes as shown below:

• Immobilized Ficin, Cat. No. 44881
• Immobilized Papain, Cat. No. 20341
• Immobilized Pepsin, Cat. No. 20343

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many Thermo Scientific IgG Fragmentation kits are available and which enzymes are included?

There are six Thermo Scientific IgG fragmentation kits:

• Fab Preparation Kit (Papain), Cat. No. 44985
• Fab Micro Preparation Kit (Papain), Cat. No. 44685
• F(abÁ)2 Preparation Kit (Pepsin), Cat. No. 44988
• F(abÁ)2 Micro Preparation Kit, (Pepsin), Cat. No. 44688
• Mouse IgG1 Fab and F(abÁ)2 Preparation Kit (Ficin), Cat. No. 44980
• Mouse IgG1 Fab and F(abÁ)2 Micro Preparation Kit (Ficin), Cat. No. 44680

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.