Ion 文库 TaqMan™ 定量试剂盒
Ion 文库 TaqMan™ 定量试剂盒
Ion Torrent™

Ion 文库 TaqMan™ 定量试剂盒

Ion 文库 TaqMan™ 定量试剂盒是一种易于使用的灵活解决方案，用于 Ion Torrent 半导体测序工作流程中文库输入的精确定量检测。该综合试剂盒提供了 TaqMan™ qPCR了解更多信息
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货号数量
44688022 个管
货号 4468802
价格（CNY)
23,915.00
Each
添加至购物车
数量:
2 个管
价格（CNY)
23,915.00
Each
添加至购物车
Ion 文库 TaqMan™ 定量试剂盒是一种易于使用的灵活解决方案，用于 Ion Torrent 半导体测序工作流程中文库输入的精确定量检测。该综合试剂盒提供了 TaqMan™ qPCR 混合物和文库标准品以检测和定量测定 Ion 片段文库中飞摩尔水平含量。使用能够快速和标准循环的通用循环条件，Ion 文库 TaqMan 定量试剂盒可支持大多数实时平台。与非探针方法相比，此通用试盒可实现更高的准确度和特异性，并使 PGM™ 系统测序工作流程更简便快速。

使用 Ion 文库 TaqMan 定量试剂盒可实现：

• 精确— TaqMan™ 分析相较于非探针定量方法在检测经适当调整的可扩增模板方面具有更高的特异性和准确度。
• 灵敏— 该方案支持对片段文库的飞摩尔水平含量进行定量检测。
• 简单性— 经验证的即用型 qPCR 标准品仅需简单系列稀释即可测定用于模板微珠制备的极佳文库浓度。
• 多功能性— 高度稳健的 qPCR 混合物可兼容多种循环条件和反应体积，并可将高灵敏度的检测与广泛定量范围相结合。
• 效率— 基于 qPCR 的方法可使用更少的材料进行定量检测。

PGM™ 系统上使用的定量文库起始量
Ion 文库 TaqMan 定量试剂盒可使 Ion 片段文库中的可扩增模板定量用于 Ion Torrent 测序工作流程中的模板制备。该试剂盒包括设计用于 Ion 文库接头和 Ion 文库对照品的定制 TaqMan™ qPCR 混合物，是确定高质量测序结果所需较佳起始材料含量的完整解决方案。

本试剂盒包括：

• 设计用于 Ion 文库接头的 Custom TaqMan™ 测定试剂盒
• 经过优化的 Ion 文库扩增混合物
• 使用 Ion 片段文库试剂盒以规定尺寸构建的经调整大肠杆菌 DH10B 文库对照品以建立一个大小为 ∼190 bp 的文库，提供质量控制所需的基本组分，同时保持灵活性以满足您的实验室需求。

与多种应用和仪器兼容
借助 Ion 文库 TaqMan 定量试剂盒，能够使用大多数使用快速或标准循环程序的实时仪器来准确定量基因组和扩增子文库。这些仪器包括 Applied Biosystems 7900HT、7300、7500、ViiA 7、StepOne™ 和 StepOnePlus™ 系统。已在 Ion 片段文库定量检测方面对该试剂盒进行了验证。建议将试剂盒中所含 Ion 特异性大肠杆菌 DH10B 文库对照品仅用作 qPCR 对照品而不是测序对照品。
仅供科研使用。不可用于诊断程序。
规格
校准类型测序 - 文库定量检测
发射可见光
激发可见光
适用于（应用）测序
适用于（设备）Ion PGM™ 系统和 Ion S5 Plus
反应次数250
产品线Ion Torrent™
产品类型定量测定试剂盒
数量2 个管
样品体积2.5 ml
运输条件湿冰
工作流程步骤文库定量检测
产品规格试剂盒
Unit SizeEach
内容与储存
将组成部分储存在 -20°C 下

常见问题解答 (FAQ)

If adapter dimers are present in my library, can they be detected using the Ion Library TaqMan Quantitation Kit (qPCR)?

No, the Ion Library TaqMan Quantitation Kit for qPCR library quantification is unable to differentiate amplifiable primer-dimers from library fragments. We recommend assessing the library size distribution, including checking for the presence of adapter dimers, using the Bioanalyzer instrument. Libraries containing adapter dimers will have sharp peaks at ~70 bp for non-barcoded libraries or ~90 bp for barcoded libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle?

The Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle (Cat. No. A46497) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, the IonCode Barcode Adapters, the Ion 550 Kit - Chef, and the Ion 550 Chip Kit for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 384 research samples.
Note: The Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) must be purchased separately.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion AmpliSeq Microbiome Health Research Kit, Library bundle?

The Ion AmpliSeq Microbiome Health Research Kit, Library bundle (Cat. No. A46495) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, and the IonCode Barcode Adapters for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 48 research samples.
Note: The Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) must be purchased separately.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I use the AmpliSeq_Microbiome_Health_ Analysis plugin for Torrent Suite Software and Ion Reporter Software workflows for my custom 16S or target species AmpliSeq panel?

The Torrent Suite AmpliSeq_Microbiome_Health_ Analysis plugin and Ion Reporter Software workflows were developed and validated exclusively for the Ion AmpliSeq Microbiome Health Research Kit. Open source analysis tools may be available for your custom solutions.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I customize the list of target species for my microbiome diversity research project?

Customization solutions are available through AmpliSeq custom designs (Ampliseq.com), where you can customize your own assays for any species of your interest. For details, please contact your local Field Applications Scientist (FAS).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

View all Ion 文库 TaqMan™ 定量试剂盒 FAQs