QuantStudio™ 12K Flex OpenArray™ Block RNase P Kit - FAQs

What is the purpose of the RNase P Detection Reagents Kit (Cat. No. 4316831), and what is provided in the kit?

RNase P is a single-copy gene encoding the RNA moiety for the RNase P enzyme, and it is an excellent gene to use as a reference/control for Copy Number Variation (CNV) assays. The TaqMan RNase P Detection Reagents Kit contains a 20X mix of primers and probe (FAM dye-labeled) that will detect and quantitate genomic copies of the human RNase P gene. The primers and probe are designed according to TaqMan chemistry guidelines for quantitation, and utilize the universal thermal cycling parameters. This kit is used to perform a 5' nuclease assay with TaqMan Universal PCR Master Mix (Cat. No. 4304437) or the TaqMan Core PCR Reagents Kit (Cat. No. N808-0228) with genomic, plasmid or complementary DNA (cDNA) templates.

The complete components of the TaqMan RNase P Detection Reagents Kit, Cat. No. 4316831, are: 20X RNase P Primer-Probe (FAM/TAMRA probe) Mix, 1 tube Human Genomic Control DNA, 10 ng/ul (100 ul), and the Product Insert with an overview and procedure for the use of the kit.

What type of Real-Time PCR (Sequence Detection System) information is available from Applied Biosystems?

Tutorials demonstrating introductions to Real-Time PCR, guidelines for designing real-time assays using Primer Express software, and various instrument set-ups can be found under Life Technologies University on our website. User bulletins, manuals and Product inserts, protocols can be found as downloadable pdf files on our Technical Resources page.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.