Peroxisome proliferator-activated receptor gamma ligand troglitazone induces cell cycle arrest and apoptosis of hepatocellular carcinoma cell lines.
AuthorsYoshizawa K, Cioca DP, Kawa S, Tanaka E, Kiyosawa K,
JournalCancer
PubMed ID12412180
'Ligand activation of peroxisome proliferator-activated receptor gamma (PPARgamma) results in the inhibition of proliferation of various cancer cells. The aim of this study is to investigate the mechanisms of cell growth inhibition of hepatocellular carcinoma (HCC) cell lines by the PPARgamma ligand, troglitazone. Six HCC cell lines were used to ... More
Development of immunomodulatory six base-length non-CpG motif oligonucleotides for cancer vaccination.
'We have previously described a novel family of immunomodulatory synthetic oligonucleotides characterized by a phosphodiester backbone, a length of six bases and a 5''G3xG23'' sequence, where x is A, C, G or T. In the present study, we have evaluated whether these 5''G3xG23'' oligonucleotides possess additional activities essential for adequate ... More
Induction of apoptosis and CD10/neutral endopeptidase expression by jaspamide in HL-60 line cells.
AuthorsCioca DP, Kitano K,
JournalCell Mol Life Sci
PubMed ID12363040
'Jaspamide (asplakinolide) is a natural peptide isolated from marine sponges of Jaspis species and has fungicidal and growth-inhibiting activities. We characterized the jasplakinolide-induced loss of viability by programmed cell death in the HL-60 human promyelocytic leukemia cell line and found that this process was accompanied by neutral endopeptidase (NEP)/CD10 expression ... More
Disordered IL-33/ST2 activation in decidualizing stromal cells prolongs uterine receptivity in women with recurrent pregnancy loss.
AuthorsSalker MS, Nautiyal J, Steel JH, Webster Z, Sucurovic S, Nicou M, Singh Y, Lucas ES, Murakami K, Chan YW, James S, Abdallah Y, Christian M, Croy BA, Mulac-Jericevic B, Quenby S, Brosens JJ,
JournalPLoS One
PubMed ID23300625
Decidualization renders the endometrium transiently receptive to an implanting blastocyst although the underlying mechanisms remain incompletely understood. Here we show that human endometrial stromal cells (HESCs) rapidly release IL-33, a key regulator of innate immune responses, upon decidualization. In parallel, differentiating HESCs upregulate the IL-33 transmembrane receptor ST2L and other ... More
Skin cells and tissue are capable of using L-ergothioneine as an integral component of their antioxidant defense system.
AuthorsMarkova NG, Karaman-Jurukovska N, Dong KK, Damaghi N, Smiles KA, Yarosh DB,
JournalFree Radic Biol Med
PubMed ID19439218
The cellular defense system against harmful levels of reactive oxygen species consists of antioxidant enzymatic activities and small nonenzymatic molecules. L-ergothioneine has long been recognized as a potent and stable low-molecular-weight antioxidant that humans consume with diet and that accumulates in cells normally subjected to high levels of oxidative stress. ... More
Genistein inhibits radiation-induced activation of NF-kappaB in prostate cancer cells promoting apoptosis and G2/M cell cycle arrest.
AuthorsRaffoul JJ, Wang Y, Kucuk O, Forman JD, Sarkar FH, Hillman GG,
JournalBMC Cancer
PubMed ID16640785
New cancer therapeutic strategies must be investigated that enhance prostate cancer treatment while minimizing associated toxicities. We have previously shown that genistein, the major isoflavone found in soy, enhanced prostate cancer radiotherapy in vitro and in vivo. In this study, we investigated the cellular and molecular interaction between genistein and ... More
Proliferation marker pKi-67 affects the cell cycle in a self-regulated manner.
AuthorsSchmidt MH, Broll R, Bruch HP, Duchrow M,
JournalJ Cell Biochem
PubMed ID12397615
The proliferation marker pKi-67 is commonly used in research and pathology to detect proliferating cells. In a previous work, we found the protein to be associated with regulators of the cell cycle, controlling S-phase progression, as well as entry into and exit from mitosis. Here we investigate whether pKi-67 has ... More
RNA interference is a functional pathway with therapeutic potential in human myeloid leukemia cell lines.
AuthorsCioca DP, Aoki Y, Kiyosawa K,
JournalCancer Gene Ther
PubMed ID12536201
RNA interference (RNAi) is a cellular pathway of gene silencing in a sequence-specific manner at the messenger RNA level. The basic mechanism behind RNAi is the breaking of a double-stranded RNA (dsRNA) matching a specific gene sequence into short pieces called short interfering RNA, which trigger the degradation of mRNA ... More
Induction of apoptosis in human lung cancer cells by curcumin.
Curcumin, a phenolic compound from the rhizome of the plant Curcuma longa has anti-inflammatory, antioxidant and anti-cancer activities. Although the precise mode of action of this compound is not yet elucidated, studies have shown that chemo-preventive action of curcumin might be due to its ability to induce apoptosis and to ... More
Depletion of alveolar macrophages exerts protective effects in pulmonary tuberculosis in mice.
AuthorsLeemans JC, Juffermans NP, Florquin S, van Rooijen N, Vervoordeldonk MJ, Verbon A, van Deventer SJ, van der Poll T,
JournalJ Immunol
PubMed ID11254718
Mycobacterium tuberculosis bacilli are intracellular organisms that reside in phagosomes of alveolar macrophages (AMs). To determine the in vivo role of AM depletion in host defense against M. tuberculosis infection, mice with pulmonary tuberculosis induced by intranasal administration of virulent M. tuberculosis were treated intranasally with either liposome-encapsulated dichloromethylene diphosphonate ... More