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TaqMan™ OpenArray™ Genotyping Plate, Custom Format 180 QuantStudio™ 12K Flex - FAQs

查看更多产品信息 TaqMan™ OpenArray™ Genotyping Plate, Custom Format 180 QuantStudio™ 12K Flex - FAQs (4471117)

6 个常见问题解答

Why is there not a format 32 for custom Gene Expression OpenArrays like there are for custom Genotyping OpenArrays?

The format differences are due to the use of start and stop points. These are not used for GX arrays. In format 32, the 2 sample/subarray format is loaded mirrored. If we did this for GX, we would only end up being able to put assays in columns 1-4 of the subarray (5-6 contains the start points and 7-8 the stop points). The result would be just 16 assays. These are not made as the format was not deemed useful.

Find additional tips, troubleshooting help, and resources within our TaqMan Protein Assay Support Center.

What type of Real-Time PCR (Sequence Detection System) information is available from Applied Biosystems?

Tutorials demonstrating introductions to Real-Time PCR, guidelines for designing real-time assays using Primer Express software, and various instrument set-ups can be found under Life Technologies University on our website. User bulletins, manuals and Product inserts, protocols can be found as downloadable pdf files on our Technical Resources page.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What are the cycling conditions for OpenArray off-line cycling on a ProFlex PCR System?

These instructions may be found on page 79 of the ProFlex PCR System User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0007697.pdf).

Reaction volume: 33 nL
Cover temperature: 70°C

94°C for 600 sec, ramp rate 1.3

50 cycles of:
99°C for 30 seconds, ramp rate 1.3
54°C for 21 seconds, ramp rate 1.1
57°C for 90 seconds, ramp rate 0.1

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What are the cycling conditions for OpenArray off-line cycling on a GeneAmp PCR System 9700?

These instructions may be found on page 5 of the Dual Flat Block GeneAmp PCR System 9700 User Guide (https://tools.thermofisher.com/content/sfs/manuals/cms_065075.pdf).

93°C for 10 min, ramp rate 100%

50 cycles of:
95°C for 45 sec, 84% ramp rate
94°C for 13 sec, 100% ramp rate
53°C for 2 min 14 sec, 44% ramp rate

25°C for 2 min, 100% ramp rate

Optional: Add a post-PCR overnight hold as shown below:
4°C for 99 min 59 sec, 100% ramp rate

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What additional accessories do I need to perform off-line cycling for QuantStudio OpenArray plates?

You need an OpenArray Plate Adaptor, Cat. No. 4472306. You do not need a compression pad. This part number works with both the GeneAmp PCR System 9700 and the ProFlex PCR System.
Please note that this part number does not have a product page on our website. To order, please call our customer service team at 800-955-6288, option 1, option 1.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How can I get additional throughput for the QuantStudio 12K Flex OpenArray System?

Additional OpenArray plates can be run off-line using a flat-block thermal cycler. The two thermal cyclers we recommend are the GeneAmp PCR System 9700 and the ProFlex PCR System.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.