Ion Plus 片段文库试剂盒
Ion Plus 片段文库试剂盒
Ion Torrent™

Ion Plus 片段文库试剂盒

作为 Personal Genome Machine™ 测序平台的一个一体式组成部分,Ion Plus 片段文库试剂盒设计用于通过多个可能的工作流程生成高质量 DNA 文库。经济实惠的了解更多信息
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货号 4471252
价格(CNY)
-
作为 Personal Genome Machine™ 测序平台的一个一体式组成部分,Ion Plus 片段文库试剂盒设计用于通过多个可能的工作流程生成高质量 DNA 文库。经济实惠的 Ion Plus 片段文库试剂盒提供了低成本的样品制备,可在每个实验室进行下一代测序。这款革命性试剂盒可结合物理剪切方法或我们专有的 Ion Shear™ 酶促片段化技术(Ion Xpress Plus 文库试剂盒,货号 4471269),在短短2小时内完成 gDNA 和扩增子文库的文库制备,从而可节省时间和成本。相对于其他文库构建技术,其产量明显更高,只需 100 ng 的 DNA 起始量即可获得高质量的文库。此外,这一高效的文库构建过程使您能够使用 <1 μg 的起始材料创建“无扩增”文库。这款试剂盒还非常灵活,可与多种物理剪切方法和文库规格选择方法兼容。

Ion Plus 片段文库试剂盒含有样品制备试剂,可针对多达 20 种 DNA 文库(取决于输入 DNA 类型和数量)进行文库构建,用于半导体测序。在 -20°C 下储存组分。

Ion Plus 片段文库试剂盒可提供:
• 起始量更低,起始材料可低至 100 ng
• 快速而灵活的工作流程,生产含有不同大小插入片段的 Ion 片段文库,并提供可选的混合和匹配工作流程
• 适用于自动化文库制备系统(包括 AB Library Builder™ 系统)的可扩展方法

单日工作流程:测序革命的下一阶段
Ion Plus 片段文库试剂盒的周转时间较短,可将 Ion 测序工作流程缩短至单个工作日,让您能够在比以往更短的时间内完成对样品的全面分析。此外,Ion Plus 片段文库试剂盒可与多种 DNA 剪切和文库规格选择方法兼容,可使您根据项目需求定制工作流程。

仅供研究使用。不得用于人或动物的治疗或诊断用途。
For Research Use Only. Not for use in diagnostic procedures.
规格
适用于(应用)Post-Light™ Ion 半导体测序
适用于(设备)Ion PGM™ 系统、Ion OneTouch™ 系统
高通量能力兼容高通量应用,多通路(技术)
片段文库
产品线Ion Torrent™, Ion Xpress™
数量10 次反应
样品类型DNA
测序类型基因组 & DNA 测序
运输条件干冰
工作流程步骤文库生成
Unit SizeEach

常见问题解答 (FAQ)

What are the recommended conditions for long-term storage of my libraries?

For long-term storage, libraries should be stored concentrated (dilutions recommended for template preparation are not intended for long term storage) in LoBind tubes in low TE in non-frost free freezer at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

If adapter dimers are present in my library, can they be detected using the Ion Library TaqMan Quantitation Kit (qPCR)?

No, the Ion Library TaqMan Quantitation Kit for qPCR library quantification is unable to differentiate amplifiable primer-dimers from library fragments. We recommend assessing the library size distribution, including checking for the presence of adapter dimers, using the Bioanalyzer instrument. Libraries containing adapter dimers will have sharp peaks at ~70 bp for non-barcoded libraries or ~90 bp for barcoded libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

I tried quantifying my amplified library with both the Ion Library Quantitation Kit (qPCR) and the Bioanalyzer instrument. The concentration according to qPCR is lower than the Bioanalyzer instrument. Which concentration should I use?

We recommend using the qPCR result, as quantification by qPCR is generally more accurate than quantification using the Bioanalyzer instrument.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can the Ion Library Equalizer Kit (Cat. No. 4482298) be used to normalize 400-base read libraries?

Currently, the Ion Library Equalizer Kit is validated and only recommended for use with up to 300-base read libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

For quantification of my library on the Bioanalyzer instrument, do I have to amplify my library first?

We highly recommend quantifying unamplified libraries using qPCR, as it specifically measures the amplifiable (or usable) library fragments present. Unamplified libraries will contain unadapted and improperly adapted fragments, which will be included in quantification data obtained if using the Bioanalyzer instrument. Library quantification on the Bioanalyzer instrument using the Agilent High Sensitivity DNA Kit is only recommended for amplified libraries, as the library amplification process selects for properly adapted library fragments.

The Bioanalyzer instrument can be used to assess the size distribution of both amplified and unamplified libraries. Please see the Ion Xpress Plus gDNA Fragment Library Preparation User Guide (Pub. No. 4471989) for detailed information. If using the Bioanalyzer instrument for quantification of unamplified libraries, please note that this method will generally underestimate the concentration of usable library fragments and a library input titration may be necessary to optimize template preparation.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.