Ion Torrent™

Ion Xpress™ 条形码接头 49-64 试剂盒

货号	数量
4474519	1 kit

货号 4474519

价格（CNY)

1 kit

The Ion Xpress™ Barcode Adapters 49-64 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Barcode Adapters kits allows pooling of up to 96 fragment libraries.

The Ion Xpress™ Barcode Adapters 49-64 Kit 可提供一套 16 个经专门设计和验证的独特条形码适配体，可与 Ion Torrent PGM™ 和 Proton™ 半导体测序仪配套使用实现理想性能。当与 Ion Xpress™ Plus 片段文库试剂盒或 Ion Plus 片段文库试剂盒配合使用时，该试剂盒可使用户在油包水 PCR 前混合至多 16 个片段文库，然后进行多重测序分析，从而简化 Ion 半导体测序工作流程，实现包括靶向富集在内的多种应用。该试剂盒与其他条形码适配体套件一起使用，可允许混合多达 96 个片段文库。

关键产品特性：

•使用强大的分子条形码，支持在单个测序芯片上对大量片段文库样品进行多通路分析
•序列和流式经过优化，以便在池中平等显示所有条形码且以更经济的方式进行多通路测序运行
•通过设计增加对样品识别的可信度，以实现稳定的错误校正内置功能

采用条形码适配体的多通路操作可实现更高通量
采用条形码文库的多通路操作可实现每次运行多达 96 份样品（该试剂盒可运行 16 份样品），从而获得更具成本效益的运行，显著降低了 emPCR、富集和测序的成本和处理要求。

经序列和流式优化以提高性能和效率
条形码适配体包含经过优化的序列，以在池中平等显示所有条形码，仅需极低数量的流量进行研究，适配体测序只需要 17 个碱基就能实现更经济的多通路测序运行。

经设计稳定的错误校正内置功能
内置的错误校正功能几乎消除了样品分配错误的可能性。

仅供研究使用。不得用于人或动物的治疗或诊断用途。

仅供科研使用。不可用于诊断程序。

适用于（设备）Ion PGM™ 系统、Ion OneTouch™ 系统

库片段文库

产品类型条形码接头试剂盒

数量1 kit

测序类型基因组 & DNA 测序

运输条件干冰

通量96 份样品/运行

工作流程步骤文库条形码

产品线Ion Torrent

原始材料DNA

Unit SizeEach

内容与储存

1 盒 17 管；储存于 -20°C 下

常见问题解答 (FAQ)

Do libraries constructed with the Ion Xpress Barcode adapters require balancing to help ensure equal representation on the Ion PGM or Proton instrument sequencing run?

No, the Ion Xpress Barcode adapters are designed with a 3 bp adapter sequence (GAT) at the 3' end of each barcode to help minimize adapter ligation bias. When pooling barcoded libraries, accurate library quantification is extremely important to help ensure even barcode representation on the sequencing run.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many fragment or amplicon libraries can be made per barcode with the Ion Xpress Barcode Adapters kits?

The Ion Xpress Barcode Adapters kits are available as a set of 96 (Cat. No. 4474517) or in sets of 16 (Cat. Nos.4471250, 4474009, 4474518, 4474519, 4474520 or 4474521). Each barcode kit is sufficient for preparing ? 10 libraries per barcode (10 x 16 libraries or 10 x 96 libraries) for 100 ng input, or 2 libraries per barcode for 1 µg input.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Proton System?

The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section “Strategies for combining Ion Ampliseq libraries.”

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

I am planning to use the Oncomine BRCA Research Assay, Manual Library Preparation (Cat. No. A32840). Which other products would need I to order?

Here are the products you would need to order:

- Ion Xpress Barcode Adapters Kits
- Agencourt AMPure XP (Beckman Coulter Cat. No. A63880 or A63881)
- Ion Library Equalizer Kit (Cat. No. 4482298)