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查看更多产品信息 Ion OneTouch™ 2 System - FAQs (4474779)
23 个常见问题解答
The "time remaining" is not entirely accurate. The centrifuge may keep rotating for about 15 minutes after the time has become 0:00. If it rotates for more than 15 minutes, please contact Technical Support.
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If you have not used the instrument for more than 2 weeks, cleaning is recommended. You can use the Amplification Plate without changing it for your sample run. Also, please do not discard the Cleaning Adaptor, since you will use it for the cleaning after the run.
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The pressure inside the instrument may be above normal. If the Ion OneTouch 2 firmware is older than v540, please update it to the latest version.
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No, you may not. Please exchange the oil when switching between different kits. Contact Technical Support for any needed assistance when switching kits.
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It may be rubber from the packing. The amount varies among instruments and it is not anything to be concerned about, as it will be removed by the Ion OneTouch ES.
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No, you cannot. Please exchange the oil when switching between different kits. Please contact Technical Support for detailed information when switching kits.
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Open the chip clamp and check for any leaks. If you see solution leaking, please remove the chip and clear the solution with a Kimwipe. DO NOT wipe or scrub as the socket could be damaged. Clean the socket by gently touching it with a damp Kimwipe. Make sure the socket is dry and replace the chip. Press the 'Calibration' button to recalibrate the chip. If it fails again, change the chip to a different one. Also, if you are using an Ion 316 Chip v2 on a v3.4 or older instrument, the instrument will not recognize the chip.
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Template preparation for the Ion Proton System requires the Ion OneTouch 2 Instrument (Cat. No. 4474779). Please contact your sales representative for more information regarding Ion OneTouch 2 System upgrade promotions for current Ion OneTouch Instrument users.
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Downloading log files may be necessary to help a Support Specialist diagnose an instrument issue. In order to download the log files, attach an empty USB drive to your instrument, navigate to the ‘Options' menu, and press the ‘Export Log' button. Wait for the indicator to complete, remove your drive, and send the log files to your Support Specialist. Please note that the Ion OneTouch 2 instrument log files are saved in temporary memory and will be erased if the instrument is power-cycled.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
Firmware updates to the software controlling the Ion OneTouch 2 Instrument are periodically released. To update the firmware to the appropriate version, use either a USB flash drive or an ethernet connection, as described on page 11 of the Ion OneTouch 2 System - Installation, Setup, and Maintenance User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0014388_IonOneTouch2Sys_UG.pdf ).
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Please perform the residual volume test as outlined in the Appendix of your template user guide. If the volume in Well 2 is >5 µL, then proceed to recalibrate the instrument following the guidelines in the “Calibrate the Ion OneTouch ES” section. Only perform a calibration if the residual volume test indicates it is necessary.
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We recommend removing the samples from the Ion OneTouch 2 instrument ≤ 16 hours after starting the run. After centrifuging and removing the samples from the instrument, follow the ISP collection protocol to recommended stopping points and follow the instructions for preparing the unenriched ISPs for storage. The ISP suspension can be stored at 2 degrees C to 8 degrees C for up to 3 days.
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Yes, there are three supported options:
• Ion Sphere Quality Control Kit (Cat. No. 4468656) for use with the Qubit 2.0 or 3.0 Fluorometer - This is the most commonly used templated ISP quality control method. This kit supports the quality control of unenriched ISPs and the protocol can be found in the Appendix of all Ion Template kit user guides.
• ISP quality control using flow cytometry:
- Ion Sphere Particles Quality Assessment Using the Guava easyCyte 5 Flow Cytometer (http://ioncommunity.thermofisher.com/docs/DOC-2494)br/>
- Demonstrated Protocol: Ion Sphere Particles (ISPs) Quality Assessment using the Applied Biosystems Attune Acoustic Focusing Cytometer (http://ioncommunity.thermofisher.com/docs/DOC-2790)
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In a typical Ion OneTouch 2 run, you should expect ~18 mm drop in the level of oil in the tube (~22 mL consumed), and ~13 mm drop in the recovery solution (~15 mL consumed). During cleaning, you should expect to collect ~10 mL of oil in the 50 mL conical waste collection tube.
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Due to differences in reagent formulations between kit types, we recommend following the Demonstrated Protocol: How to Set Up the Ion OneTouch 2 Instrument When Switching Between the Ion PGM System and Ion Proton System (http://ioncommunity.thermofisher.com/docs/DOC-7265) if using more than one Ion OneTouch 2 Template kit type on your instrument. Briefly, the instrument lines must be primed with the new kit reagents by running a cleaning and initialization procedure prior to setting up the next run. Please see the above user guide for detailed instructions.
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The input amount for template preparation on the Ion OneTouch 2 is library type specific. Occasionally, library input titration may be required to optimize templated ISP clonality and maximize sequencing throughput. Please see the specific guidelines for your library type in the appropriate library preparation user guide that can be found here: http://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/next-generation-sequencing-support-center/ngs-library-preparation-support.html
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For the Ion PGM System, we offer:
- Ion PGM Hi-Q View OT2 Kit (Cat. No. A29900) for template preparation of libraries up to 400 bp in length.
For the Ion Proton System, we offer:
- Ion PI Hi-Q OT2 200 Kit (Cat. No. A26434) for template preparation of libraries up to 200 bp in length.
For the Ion S5 System and Ion S5 XL System, we offer two kits:
- Ion 520/530 Kit-OT2 (Cat. No. A27751) for template preparation of libraries up to 400 bp in length.
- Ion 540 Kit- OT2 (Cat. No. A27753) for template preparation of libraries up to 200 bp in length.
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The Ion OneTouch 2 System is composed of the Ion Onetouch 2 instrument and the Ion OneTouch ES instrument.
• The Ion Onetouch 2 instrument is used for templated Ion Sphere Particle (ISP) preparation. The input is a prepared library with Ion Torrent System specific adapters. The output is a mixture of templated library ISPs and non-templated ISPs (this mixture is referred to as unenriched ISPs).
• The Ion OneTouch ES instrument is used for enrichment of the templated library ISPs, or the removal of non-templated ISPs. The input is unenriched ISPs recovered from the Ion OneTouch 2 instrument and the output is templated library ISPs, or enriched ISPs, ready to be sequenced on the Ion PGM or Proton instruments.
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Currently, the Ion PGM System supports up to 400–base reads.
For up to 400-base reads, using the Ion PGM Hi-Q View Chemistry and Ion PGM One Touch 2 system, we recommend:
• Ion PGM Hi-Q View One Touch 2 Kit (Cat. No. A29900)
• Ion PGM Hi-Q View Sequencing Kit (Cat. No. A30044)
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The calibration standard is an optional reagent and is recommended for samples that have no reference or small references. It is a small panel consisting of known sequence containing homopolymers. It provides a reference for base calibration but the option needs to be selected in run planning.
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The Ion PGM Hi-Q View OneTouch 2 Kit includes the reagents required for preparing 8 reactions of template-positive Ion PGM Hi-Q View Ion Sphere Particles (ISPs) on the Ion OneTouch 2 System. The components are:
- Ion PGM View OneTouch 2 Supplies
- Ion PGM Hi?Q View OneTouch 2 Reagents
- Ion PGM Hi?Q View OneTouch 2 Solutions
- Ion PGM Calibration Standard
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
In a typical Ion OneTouch 2 run, you should expect ~18 mm drop in the level of oil in the tube (~22 mL consumed), and ~13 mm drop in the recovery solution (~15 mL consumed). During cleaning, you should expect to collect ~10 mL of oil in the 50 mL conical waste collection tube.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
Ion Torrent OneTouch2 protocols can be found at our NGS Support Center (thermofisher.com/ngssupport), in the NGS Template Preparation Support Center section. For all other protocols, please go here (http://www.thermofisher.com/manuals-protocols-search.html).
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.