Ion PI™ Chip Kit v2 - FAQs

查看更多产品信息 Ion PI™ Chip Kit v2 - FAQs (4482321)

5 个常见问题解答

What chips are available for the Ion Proton System and what are the specifications?

Currently, the Ion Proton System supports the Ion PI Chip Kit v2 (Cat. No. 4482321), which contains 165 million wells and typically generates up to 10 Gb of data and ~60-80 million reads.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Proton System?

The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section “Strategies for combining Ion Ampliseq libraries.”

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I use an Ion PI v2 chip for initializing a run using Ion PI Hi-Q sequencing chemistry?

Yes, an Ion PI v2 chip can be used for the initialization. Ensure that the Ion PI Hi-Q initialization program is selected, and an Ion PI Chip v3 is used for the sequencing run.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Is the Ion PI Chip v2 compatible with Ion PI Hi-Q sequencing chemistry?

No, the Ion PI Hi-Q sequencing chemistry is only compatible with the Ion PI Chip v3. Using the Ion PI Chip v2 will result in lower loading and data throughput.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What Ion PGM chips are available? How much sequencing data can I expect from each chip type?

The Ion PGM System supports the Ion 3 series chips, including the Ion Torrent Ion 314 Chip Kit v2 (Cat. No. 4482261), Ion Torrent Ion 316 Chip Kit v2 (Cat. Nos. 4483188 and 4483324), and Ion Torrent Ion 318 Chip Kit v2 (Cat. Nos. 4484354 and 4484355). The Ion 314 Chip Kit v2 is available in an 8-pack; the Ion 316 Chip Kit v2 and Ion 318 Chip Kit v2 are available in 4-packs and 8-packs.

For more details, please see the Ion PGM System Specification Sheet (https://tools.thermofisher.com/content/sfs/brochures/PGM-Specification-Sheet.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.