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View additional product information for ProFlex™ PCR System, 2 x 384-well - FAQs (A30163, A30164, 4484077, A30229)
19 product FAQs found
如果使用了错误的塑料耗材，或者没有使用正确的托盘/固定器，可能会发生这种情况。请查看说明书中关于正确上样的指南部分。此外，您还可以从这里(https://www.thermofisher .com/us/en/home/lifescience/pcr/pcr-plastics.html)查看兼容的塑料耗材：
Veriti热循环仪 - 上样(手册第3-5页)
ProFlex PCR系统 -上样(手册第25页)
2720热循环仪 - 上样(手册第3-5页)
如需设定一个恒定温度，请在程序中选择无限（∞) 标识。
在ProFlex PCR热循环仪中，96孔与3x32孔模块均装备了创新的VeriFlex模块用于提升PCR功能。六个分离的Peltier模块（在3x32孔模块中，-每1个模块（32孔）具有2个Peltier模块）提供最大的配置多样性和灵活性，同时提供两个关键优势：
1.PCR条件优化：每一模块均可单独设置一个特定温度，非常适合用户在PCR条件优化过程中对温度进行精确控制。
2.运行更多实验条件：六个Peltier模块也适用于在同一反应中设定六个不同的退火温度。
Auto Delta特性能够帮助您在循环阶段中逐渐增高或降低每一循环某一步骤的温度。其中一个常见应用就是降落PCR（touchdown PCR）。
96孔和3x32孔系统拥有6.0°C/秒的最大模块变温速率，双96孔和双平板模块的最大模块变温速率为2.0°C/秒。
孔板与塑料耗材均为特定模块类型所专用。有关您的模块类型兼容的塑料耗材列表，请参阅 此表。
ProFlex PCR仪可采用四种不同的模块，只需轻按开关即可进行更换。这其中也包括首创的3x32孔独立模块。这一模块能够同时运行三个完全独立、互不干扰的实验。双96孔模块用于匹配您的高通量测序需求，双平板模块支持OpenArray平板技术，用于QuantStudio 12K Flex实时荧光定量PCR系统的基因分型分析。
This can happen if the wrong plastics are used, or if the tubes are not being used with the correct tray/retainer set. Check the manual for directions on appropriate loading of the sample. Also, you can check for compatible plastics from here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html).
Veriti Thermal Cycler - Loading Samples (p. 3-5 of the manual)
ProFlex PCR System - Loading Samples (p. 25 of the manual)
2720 Thermal Cycler - Loading Samples (p. 3-5 of the manual)
To add an infinite hold, select the infinity symbol for program.
In the ProFlex PCR System, the 96-well and 3 x 32-well blocks feature innovative VeriFlex Blocks for enhanced PCR functionality. Six separate Peltier blocks (2 on each 32-well block for 3 x 32-well) provide maximal versatility and flexibility and offer two key benefits:
1.PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
2.Run more experiments: the six Peltier blocks can also be used to run up to six different annealing temperatures in the same run.
The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.
The 96-well and 3 x 32-well system have a max block ramp rate of 6.0 degrees C/sec and the dual 96-well and dual flat blocks have a max block ramp rate of 2.0 degrees C/sec.
The plates and tubes will be specific to the block type. Please refer to our PCR Tubes, Plates & Accessories Plastics Selection Guide (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html) for a list of compatible plastics, based on your block type.
The ProFlex PCR System has 4 different blocks that can be changed with the flip of a switch, including the first-of-its-kind 3 x 32-well independent block. This block allows up to 3 experiments to be run simultaneously, completely independently of each other and without interference. A dual 96-well block is available for your high throughput sequencing needs and a dual flat block is available to support OpenArray plate technology for genotyping analysis on the QuantStudio 12K Flex Real-Time PCR System.
There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.
As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.
Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf
Find additional tips, troubleshooting help, and resources within ourPCR Thermal Cyclers Support Center.
The Thermal Cycler Fleet Control Software allows monitoring of the following instruments from within a firewall:
SimpliAmp Thermal Cycler
ProFlex PCR System
Veriti Thermal Cycler
VeritiPro Thermal Cycler
Automated Thermal Cycler (ATC)
The Thermal Cycler Fleet Control Software is compatible with the following instruments:
- SimpliAmp Thermal Cycler
- ProFlex PCR System
- Veriti Thermal Cycler
- VeritiPro Thermal Cycler
- Automated Thermal Cycler (ATC)
- MiniAmp Thermal Cycler
- MiniAmp Plus Thermal Cycler
The ProFlex PCR System can be connected wirelessly with an optional High-Power USB Wi-Fi Module, Cat. No. A26774. Please note that you will need to update to the latest version of firmware in order to use this Wi-Fi module.
Additional Applied Biosystems OpenArray plates can be run off-line using a flat-block thermal cycler. The two thermal cyclers we recommend are the Applied Biosystems GeneAmp PCR System 9700 and the Applied Biosystems ProFlex PCR System.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.